3IX9
Crystal structure of Streptococcus pneumoniae dihydrofolate reductase - Sp9 mutant
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A, B | Dihydrofolate reductase | polymer | 190 | 22230.3 | 2 | UniProt (O85261) Pfam (PF00186) UniProt (by SIFTS) (Q54801) In PDB | Streptococcus pneumoniae | |
2 | A, B | NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE | non-polymer | 745.4 | 2 | Chemie (NDP) | |||
3 | A, B | METHOTREXATE | non-polymer | 454.4 | 2 | Chemie (MTX) | |||
4 | water | water | 18.0 | 306 | Chemie (HOH) |
Sequence modifications
A, B: 1 - 168 (UniProt: O85261)
PDB | External Database | Details |
---|---|---|
Met -19 | - | expression tag |
Lys -18 | - | expression tag |
His -17 | - | expression tag |
His -16 | - | expression tag |
His -15 | - | expression tag |
His -14 | - | expression tag |
His -13 | - | expression tag |
His -12 | - | expression tag |
His -11 | - | expression tag |
His -10 | - | expression tag |
Gly -9 | - | expression tag |
Gly -8 | - | expression tag |
Leu -7 | - | expression tag |
Val -6 | - | expression tag |
Pro -5 | - | expression tag |
Arg -4 | - | expression tag |
Gly -3 | - | expression tag |
Ser -2 | - | expression tag |
His -1 | - | expression tag |
Gly 0 | - | expression tag |
Tyr 26 | His 26 | engineered mutation |
Lys 60 | Gln 60 | engineered mutation |
Val 77 | Ala 77 | engineered mutation |
Ala 78 | Val 78 | engineered mutation |
His 81 | Gln 81 | engineered mutation |
Ser 91 | Gln 91 | engineered mutation |
Val 100 | Leu 100 | engineered mutation |
Ala 133 | Glu 133 | engineered mutation |
Thr 149 | Ala 149 | engineered mutation |
Leu 169 | - | expression tag |
Glu 170 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 2 |
Total formula weight | 44460.5 | |
Non-Polymers* | Number of molecules | 4 |
Total formula weight | 2399.7 | |
All* | Total formula weight | 46860.2 |