3IX9
Crystal structure of Streptococcus pneumoniae dihydrofolate reductase - Sp9 mutant
Functional Information from GO Data
Chain | GOid | namespace | contents |
A | 0004146 | molecular_function | dihydrofolate reductase activity |
A | 0005515 | molecular_function | protein binding |
A | 0005829 | cellular_component | cytosol |
A | 0006730 | biological_process | one-carbon metabolic process |
A | 0016491 | molecular_function | oxidoreductase activity |
A | 0046452 | biological_process | dihydrofolate metabolic process |
A | 0046654 | biological_process | tetrahydrofolate biosynthetic process |
A | 0046655 | biological_process | folic acid metabolic process |
A | 0046677 | biological_process | response to antibiotic |
A | 0050661 | molecular_function | NADP binding |
B | 0004146 | molecular_function | dihydrofolate reductase activity |
B | 0005515 | molecular_function | protein binding |
B | 0005829 | cellular_component | cytosol |
B | 0006730 | biological_process | one-carbon metabolic process |
B | 0016491 | molecular_function | oxidoreductase activity |
B | 0046452 | biological_process | dihydrofolate metabolic process |
B | 0046654 | biological_process | tetrahydrofolate biosynthetic process |
B | 0046655 | biological_process | folic acid metabolic process |
B | 0046677 | biological_process | response to antibiotic |
B | 0050661 | molecular_function | NADP binding |
Functional Information from PDB Data
site_id | AC1 |
Number of Residues | 32 |
Details | BINDING SITE FOR RESIDUE NDP A 193 |
Chain | Residue |
A | TRP9 |
A | VAL48 |
A | THR49 |
A | LEU66 |
A | THR67 |
A | ARG68 |
A | HIS81 |
A | VAL100 |
A | GLY101 |
A | GLY102 |
A | LYS103 |
A | ALA10 |
A | GLN104 |
A | ILE105 |
A | HOH176 |
A | HOH182 |
A | MTX200 |
A | HOH208 |
A | HOH222 |
A | HOH270 |
A | HOH275 |
A | HOH277 |
A | ILE17 |
A | HOH281 |
A | HOH297 |
A | HOH308 |
A | GLY18 |
A | ASN21 |
A | ARG22 |
A | LEU23 |
A | GLY46 |
A | ARG47 |
site_id | AC2 |
Number of Residues | 17 |
Details | BINDING SITE FOR RESIDUE MTX A 200 |
Chain | Residue |
A | ILE8 |
A | TRP9 |
A | LEU23 |
A | GLU30 |
A | GLN32 |
A | PHE34 |
A | LYS35 |
A | ARG61 |
A | VAL100 |
A | HOH187 |
A | HOH190 |
A | HOH192 |
A | NDP193 |
A | HOH206 |
A | HOH207 |
A | HOH215 |
A | HOH330 |
site_id | AC3 |
Number of Residues | 35 |
Details | BINDING SITE FOR RESIDUE NDP B 193 |
Chain | Residue |
A | ASN40 |
A | HOH185 |
A | HOH210 |
A | HOH218 |
B | TRP9 |
B | ALA10 |
B | ILE17 |
B | GLY18 |
B | LYS19 |
B | ASN21 |
B | ARG22 |
B | LEU23 |
B | TRP25 |
B | GLY46 |
B | ARG47 |
B | VAL48 |
B | THR49 |
B | LEU66 |
B | THR67 |
B | ARG68 |
B | HIS81 |
B | VAL100 |
B | GLY101 |
B | GLY102 |
B | LYS103 |
B | GLN104 |
B | ILE105 |
B | HOH182 |
B | HOH187 |
B | HOH197 |
B | MTX200 |
B | HOH205 |
B | HOH231 |
B | HOH246 |
B | HOH277 |
site_id | AC4 |
Number of Residues | 18 |
Details | BINDING SITE FOR RESIDUE MTX B 200 |
Chain | Residue |
B | NDP193 |
B | HOH236 |
B | HOH244 |
A | HOH280 |
B | ILE8 |
B | TRP9 |
B | LEU23 |
B | PRO28 |
B | GLU30 |
B | LEU31 |
B | GLN32 |
B | PHE34 |
B | LYS35 |
B | ARG56 |
B | ARG61 |
B | VAL100 |
B | THR119 |
B | HOH188 |
Functional Information from PROSITE/UniProt
site_id | PS00075 |
Number of Residues | 23 |
Details | DHFR_1 Dihydrofolate reductase (DHFR) domain signature. VIGkdnrLPWylpa.ElqhFketT |
Chain | Residue | Details |
A | VAL16-THR38 |
Functional Information from SwissProt/UniProt
site_id | SWS_FT_FI1 |
Number of Residues | 20 |
Details | BINDING: BINDING => ECO:0000250 |
Chain | Residue | Details |
A | ILE8 | |
A | THR119 | |
B | ILE8 | |
B | TRP9 | |
B | ILE17 | |
B | GLU30 | |
B | GLY46 | |
B | ARG61 | |
B | LEU66 | |
B | VAL83 | |
B | VAL100 | |
A | TRP9 | |
B | THR119 | |
A | ILE17 | |
A | GLU30 | |
A | GLY46 | |
A | ARG61 | |
A | LEU66 | |
A | VAL83 | |
A | VAL100 |