3IG8

Saccharomyces cerevisiae glutamate cysteine ligase in complex with Mg2+, L-glutamate and ADP

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Glutamate-cysteine ligase	polymer	692	79925.2	1	UniProt (P32477) Pfam (PF03074) In PDB	Saccharomyces cerevisiae (brewer's yeast lager beer yeast yeast)	Gamma-glutamylcysteine synthetase, Gamma-ECS, GCS
2	A	GLUTAMIC ACID	non-polymer		147.1	1	Chemie (GLU)
3	A	ADENOSINE-5'-DIPHOSPHATE	non-polymer		427.2	1	Chemie (ADP)
4	A	MAGNESIUM ION	non-polymer		24.3	3	Chemie (MG)
5	A	TRIETHYLENE GLYCOL	non-polymer		150.2	1	Chemie (PGE)
6		water	water		18.0	110	Chemie (HOH)

Sequence modifications

A: 1 - 678 (UniProt: P32477)

PDB	External Database	Details
Asp 679	-	expression tag
Lys 680	-	expression tag
Leu 681	-	expression tag
Ala 682	-	expression tag
Ala 683	-	expression tag
Ala 684	-	expression tag
Leu 685	-	expression tag
Gly 686	-	expression tag
His 687	-	expression tag
His 688	-	expression tag
His 689	-	expression tag
His 690	-	expression tag
His 691	-	expression tag
His 692	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	79925.2
Non-Polymers*	Number of molecules	6
	Total formula weight	797.4
All*	Total formula weight	80722.6

*Water molecules are not included.