3IG8
Saccharomyces cerevisiae glutamate cysteine ligase in complex with Mg2+, L-glutamate and ADP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 14-BM-C |
Synchrotron site | APS |
Beamline | 14-BM-C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-12-06 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 117.849, 117.849, 164.444 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 33.900 - 2.690 |
R-factor | 0.19049 |
Rwork | 0.187 |
R-free | 0.24189 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.019 |
RMSD bond angle | 1.878 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.750 |
High resolution limit [Å] | 2.690 | 2.690 |
Rmerge | 0.096 | 0.727 |
Number of reflections | 30739 | |
<I/σ(I)> | 19.4 | 2.3 |
Completeness [%] | 94.7 | 96 |
Redundancy | 7 | 5.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.8 | 291 | 12% PEG 400, pH 6.8, VAPOR DIFFUSION, SITTING DROP, temperature 291K |