3ID5

Crystal structure of Sulfolobus solfataricus C/D RNP assembled with Nop5, fibrillarin, L7Ae and a split half C/D RNA

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, E (A, E)	Pre mRNA splicing protein	polymer	388	44168.5	2	UniProt (Q97ZH3) Pfam (PF21572) Pfam (PF01798)	Sulfolobus solfataricus	Nop5
2	B, F (B, F)	Fibrillarin-like rRNA/tRNA 2'-O-methyltransferase	polymer	232	26439.4	2	UniProt (P58032) Pfam (PF01269)	Sulfolobus solfataricus	fibrillarin, FIB
3	C, G (C, G)	50S ribosomal protein L7Ae	polymer	130	14075.3	2	UniProt (P55858) Pfam (PF01248)	Sulfolobus solfataricus
4	D, H (D, H)	half C/D RNA	polymer	35	11261.7	2
5	I, J (B, F)	S-ADENOSYLMETHIONINE	non-polymer		398.4	2	Chemie (SAM)

Sequence modifications

A, E: 1 - 380 (UniProt: Q97ZH3)

PDB	External Database	Details
Val 2	Met 2	engineered mutation
His 381	-	expression tag
His 382	-	expression tag
His 383	-	expression tag
His 384	-	expression tag
His 385	-	expression tag
His 386	-	expression tag
His 387	-	expression tag
His 388	-	expression tag

B, F: 1 - 232 (UniProt: P58032)

PDB	External Database	Details
Ala 2	Ser 2	engineered mutation

C, G: 4 - 130 (UniProt: P55858)

PDB	External Database	Details
Met 1	-	expression tag
Asp 2	-	expression tag
Ala 3	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	191889.7
Non-Polymers*	Number of molecules	2
	Total formula weight	796.9
All*	Total formula weight	192686.6

*Water molecules are not included.