3ID5

Crystal structure of Sulfolobus solfataricus C/D RNP assembled with Nop5, fibrillarin, L7Ae and a split half C/D RNA

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, E (A, E)	Pre mRNA splicing protein	polymer	388	44168.5	2	UniProt (Q97ZH3) Pfam (PF21572) Pfam (PF01798)	Sulfolobus solfataricus	Nop5
2	B, F (B, F)	Fibrillarin-like rRNA/tRNA 2'-O-methyltransferase	polymer	232	26439.4	2	UniProt (P58032) Pfam (PF01269)	Sulfolobus solfataricus	fibrillarin, FIB
3	C, G (C, G)	50S ribosomal protein L7Ae	polymer	130	14075.3	2	UniProt (P55858) Pfam (PF01248)	Sulfolobus solfataricus
4	D, H (D, H)	half C/D RNA	polymer	35	11261.7	2
5	I, J (B, F)	S-ADENOSYLMETHIONINE	non-polymer		398.4	2	Chemie (SAM) PubChem (5136) PubChem (34755) PubChem (58633578) PubChem (60038769) PubChem (73897310) PubChem (58766358) PubChem (87224684) PubChem (131697735) PubChem (9865603) PubChem (145712080) PubChem (12312798) PubChem (132988922) PubChem (134692588) PubChem (135030730) PubChem (140459785) PubChem (163046094)

Sequence modifications

A, E: 1 - 380 (UniProt: Q97ZH3)

PDB	External Database	Details
Val 2	Met 2	engineered mutation
His 381	-	expression tag
His 382	-	expression tag
His 383	-	expression tag
His 384	-	expression tag
His 385	-	expression tag
His 386	-	expression tag
His 387	-	expression tag
His 388	-	expression tag

B, F: 1 - 232 (UniProt: P58032)

PDB	External Database	Details
Ala 2	Ser 2	engineered mutation

C, G: 4 - 130 (UniProt: P55858)

PDB	External Database	Details
Met 1	-	expression tag
Asp 2	-	expression tag
Ala 3	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	191889.7
Non-Polymers*	Number of molecules	2
	Total formula weight	796.9
All*	Total formula weight	192686.6

*Water molecules are not included.