3ID5
Crystal structure of Sulfolobus solfataricus C/D RNP assembled with Nop5, fibrillarin, L7Ae and a split half C/D RNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Wavelength(s) | 1.00821 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 265.485, 265.485, 129.434 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 4.010 |
| R-factor | 0.286 |
| Rwork | 0.285 |
| R-free | 0.30400 |
| Structure solution method | SIRAS |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.135 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 4.070 |
| High resolution limit [Å] | 4.000 | 4.000 |
| Rmerge | 0.139 | 0.830 |
| Number of reflections | 39544 | |
| <I/σ(I)> | 17.5 | 1.6 |
| Completeness [%] | 98.5 | 85.5 |
| Redundancy | 12.3 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 293 | 1.7M (NH4)2SO4, 50mM Na cacodylate, pH 5.6, vapor diffusion, hanging drop, temperature 293K |
