3HX0

ternary complex of L277A, H511A, R514 mutant pol lambda bound to a 2 nucleotide gapped DNA substrate with a scrunched dA

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, E, I, M (A, F, K, P)	DNA polymerase lambda	polymer	335	37252.4	4	UniProt (Q9UGP5) Pfam (PF14716) Pfam (PF10391) Pfam (PF14792) Pfam (PF14791)	Homo sapiens (Human)	Pol Lambda,DNA polymerase beta-2,Pol beta2,DNA polymerase kappa
2	B, F, J, N (B, G, L, Q)	5'-D(*CP*GP*GP*CP*AP*AP*AP*TP*AP*CP*TP*G)-3'	polymer	12	3671.4	4
3	C, G, K, O (C, H, M, R)	5'-D(*CP*AP*GP*TP*AP*T)-3'	polymer	6	1808.2	4
4	D, H, L, P (D, I, N, S)	5'-D(P*GP*CP*CP*G)-3'	polymer	4	1191.8	4
5	Q, S, V, Z (A, F, K, P)	2',3'-DIDEOXY-THYMIDINE-5'-TRIPHOSPHATE	non-polymer		466.2	4	Chemie (D3T)
6	AA, R, T, W (P, A, F, K)	MAGNESIUM ION	non-polymer		24.3	4	Chemie (MG)
7	BA, CA, U, X, Y (P, F, K)	SODIUM ION	non-polymer		23.0	5	Chemie (NA)
8	DA, EA, FA, GA, HA... (A, B, F, G, K...)	water	water		18.0	35	Chemie (HOH)

Sequence modifications

A, F, K, P: 242 - 575 (UniProt: Q9UGP5)

PDB	External Database	Details
Met 241	-	initiating methionine
Ala 277	Leu 277	engineered mutation
Ala 511	His 511	engineered mutation
Ala 514	Arg 514	engineered mutation
Ala 543	Cys 543	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	16
	Total formula weight	175695.6
Non-Polymers*	Number of molecules	13
	Total formula weight	2076.8
All*	Total formula weight	177772.4

*Water molecules are not included.