3HX0

ternary complex of L277A, H511A, R514 mutant pol lambda bound to a 2 nucleotide gapped DNA substrate with a scrunched dA

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0003677	molecular_function	DNA binding
A	0003887	molecular_function	DNA-directed DNA polymerase activity
A	0006281	biological_process	DNA repair
A	0016779	molecular_function	nucleotidyltransferase activity
A	0034061	molecular_function	DNA polymerase activity
F	0003677	molecular_function	DNA binding
F	0003887	molecular_function	DNA-directed DNA polymerase activity
F	0006281	biological_process	DNA repair
F	0016779	molecular_function	nucleotidyltransferase activity
F	0034061	molecular_function	DNA polymerase activity
K	0003677	molecular_function	DNA binding
K	0003887	molecular_function	DNA-directed DNA polymerase activity
K	0006281	biological_process	DNA repair
K	0016779	molecular_function	nucleotidyltransferase activity
K	0034061	molecular_function	DNA polymerase activity
P	0003677	molecular_function	DNA binding
P	0003887	molecular_function	DNA-directed DNA polymerase activity
P	0006281	biological_process	DNA repair
P	0016779	molecular_function	nucleotidyltransferase activity
P	0034061	molecular_function	DNA polymerase activity

Functional Information from PDB Data

site_id	AC1
Number of Residues	16
Details	BINDING SITE FOR RESIDUE D3T A 701

Chain	Residue
A	HOH7
A	PHE506
A	GLY508
A	ALA510
A	ASN513
A	MG801
B	DA6
C	DT6
A	ARG386
A	GLY416
A	SER417
A	ARG420
A	GLY426
A	ASP427
A	ASP429
A	TYR505

---

site_id	AC2
Number of Residues	4
Details	BINDING SITE FOR RESIDUE MG A 801

Chain	Residue
A	HOH7
A	ASP427
A	ASP429
A	D3T701

---

site_id	AC3
Number of Residues	17
Details	BINDING SITE FOR RESIDUE D3T F 702

Chain	Residue
F	HOH15
F	HOH16
F	ARG386
F	GLY416
F	SER417
F	ARG420
F	GLY426
F	ASP427
F	ASP429
F	TYR505
F	PHE506
F	GLY508
F	ALA510
F	ASN513
F	MG802
G	DA6
H	DT6

---

site_id	AC4
Number of Residues	4
Details	BINDING SITE FOR RESIDUE MG F 802

Chain	Residue
F	HOH15
F	ASP427
F	ASP429
F	D3T702

---

site_id	AC5
Number of Residues	5
Details	BINDING SITE FOR RESIDUE NA F 806

Chain	Residue
F	HOH12
F	SER339
F	ILE341
F	ALA344
H	DA5

---

site_id	AC6
Number of Residues	13
Details	BINDING SITE FOR RESIDUE D3T K 703

Chain	Residue
K	HOH34
K	ARG386
K	GLY416
K	SER417
K	ARG420
K	GLY426
K	ASP427
K	ASP429
K	TYR505
K	ASN513
K	MG803
K	NA808
M	DT6

---

site_id	AC7
Number of Residues	5
Details	BINDING SITE FOR RESIDUE MG K 803

Chain	Residue
K	HOH34
K	ASP427
K	ASP429
K	D3T703
K	NA808

---

site_id	AC8
Number of Residues	5
Details	BINDING SITE FOR RESIDUE NA K 807

Chain	Residue
K	HOH21
K	SER339
K	ILE341
K	ALA344
M	DT6

---

site_id	AC9
Number of Residues	5
Details	BINDING SITE FOR RESIDUE NA K 808

Chain	Residue
K	ASP427
K	ASP429
K	ASP490
K	D3T703
K	MG803

---

site_id	BC1
Number of Residues	15
Details	BINDING SITE FOR RESIDUE D3T P 704

Chain	Residue
P	HOH35
P	ARG386
P	GLY416
P	SER417
P	ARG420
P	GLY426
P	ASP427
P	ASP429
P	TYR505
P	PHE506
P	ALA510
P	ASN513
P	MG804
P	NA810
R	DT6

---

site_id	BC2
Number of Residues	4
Details	BINDING SITE FOR RESIDUE MG P 804

Chain	Residue
P	HOH35
P	ASP427
P	ASP429
P	D3T704

---

site_id	BC3
Number of Residues	5
Details	BINDING SITE FOR RESIDUE NA P 809

Chain	Residue
P	HOH29
P	SER339
P	ILE341
P	ALA344
R	DT6

---

site_id	BC4
Number of Residues	4
Details	BINDING SITE FOR RESIDUE NA P 810

Chain	Residue
P	ASP429
P	ASP490
P	D3T704
P	ASP427

---

Functional Information from PROSITE/UniProt

site_id	PS00522
Number of Residues	20
Details	DNA_POLYMERASE_X DNA polymerase family X signature. GSYrRGkatCgDVDVLIthP

Chain	Residue	Details
A	GLY416-PRO435

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	224
Details	Region: {"description":"DNA-binding","evidences":[{"source":"PubMed","id":"14992725","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	36
Details	Region: {"description":"Involved in primer binding","evidences":[{"evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	4
Details	Active site: {"description":"Schiff-base intermediate with DNA","evidences":[{"source":"PubMed","id":"11457865","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI4
Number of Residues	32
Details	Binding site: {"evidences":[{"source":"PubMed","id":"17475573","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"2PFP","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"2PFQ","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI5
Number of Residues	12
Details	Binding site: {"evidences":[{"source":"PubMed","id":"17475573","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"2PFO","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"2PFQ","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

Catalytic Information from CSA

site_id	CSA1
Number of Residues	1
Details	Annotated By Reference To The Literature 1jms

Chain	Residue	Details
A	ASP490

---

site_id	CSA2
Number of Residues	1
Details	Annotated By Reference To The Literature 1jms

Chain	Residue	Details
F	ASP490

---

site_id	CSA3
Number of Residues	1
Details	Annotated By Reference To The Literature 1jms

Chain	Residue	Details
K	ASP490

---

site_id	CSA4
Number of Residues	1
Details	Annotated By Reference To The Literature 1jms

Chain	Residue	Details
P	ASP490

---