3HTX

Crystal structure of small RNA methyltransferase HEN1

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, D (A, D)	HEN1	polymer	950	105542.5	2	UniProt (Q945R3) Pfam (PF21224) Pfam (PF18441) Pfam (PF17842) Pfam (PF13847) UniProt (by SIFTS) (Q9C5Q8)	Arabidopsis thaliana
2	B, E (B, E)	5'-R(*GP*AP*UP*UP*UP*CP*UP*CP*UP*CP*UP*GP*CP*AP*AP*GP*CP*GP*AP*AP*AP*G)-3'	polymer	22	7019.2	2
3	C, F (C, F)	5'-R(P*UP*UP*CP*GP*CP*UP*UP*GP*CP*AP*GP*AP*GP*AP*GP*AP*AP*AP*UP*CP*AP*C)-3'	polymer	22	7042.3	2
4	G, I (A, D)	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
5	H, J (A, D)	S-ADENOSYL-L-HOMOCYSTEINE	non-polymer		384.4	2	Chemie (SAH)
6	K, L, M, N, O... (A, B, C, D, E...)	water	water		18.0	37	Chemie (HOH)

Sequence modifications

A, D: 1 - 942 (UniProt: Q945R3)

PDB	External Database	Details
Met -7	-	expression tag
Gly -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
His 0	-	expression tag
Pro 604	Leu 604	engineered mutation
Lys 640	Arg 640	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	239207.9
Non-Polymers*	Number of molecules	4
	Total formula weight	817.4
All*	Total formula weight	240025.4

*Water molecules are not included.