3HTX

Crystal structure of small RNA methyltransferase HEN1

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, D (A, D)	HEN1	polymer	950	105542.5	2	UniProt (Q945R3) Pfam (PF24995) Pfam (PF21224) Pfam (PF18441) Pfam (PF17842) Pfam (PF13847) UniProt (by SIFTS) (Q9C5Q8)	Arabidopsis thaliana
2	B, E (B, E)	5'-R(*GP*AP*UP*UP*UP*CP*UP*CP*UP*CP*UP*GP*CP*AP*AP*GP*CP*GP*AP*AP*AP*G)-3'	polymer	22	7019.2	2
3	C, F (C, F)	5'-R(P*UP*UP*CP*GP*CP*UP*UP*GP*CP*AP*GP*AP*GP*AP*GP*AP*AP*AP*UP*CP*AP*C)-3'	polymer	22	7042.3	2
4	G, I (A, D)	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG) PubChem (888)
5	H, J (A, D)	S-ADENOSYL-L-HOMOCYSTEINE	non-polymer		384.4	2	Chemie (SAH) PubChem (16760024) PubChem (193) PubChem (13792) PubChem (177678424) PubChem (189149) PubChem (439155) PubChem (25210495) PubChem (21125603) PubChem (44361184) PubChem (25203782) PubChem (46936206) PubChem (50989525) PubChem (51380914) PubChem (52224398) PubChem (57544532) PubChem (52224400) PubChem (59046843) PubChem (59170628) PubChem (59576228) PubChem (66805708) PubChem (5284331) PubChem (70689219) PubChem (70702054) PubChem (70832431) PubChem (71170554) PubChem (13231890) PubChem (6419189) PubChem (118374891) PubChem (118560068) PubChem (126969526) PubChem (129105892) PubChem (164157219) PubChem (143518505) PubChem (154578335) PubChem (163054214) PubChem (163335525)
6	K, L, M, N, O... (A, B, C, D, E...)	water	water		18.0	37	Chemie (HOH)

Sequence modifications

A, D: 1 - 942 (UniProt: Q945R3)

PDB	External Database	Details
Met -7	-	expression tag
Gly -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
His 0	-	expression tag
Pro 604	Leu 604	engineered mutation
Lys 640	Arg 640	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	239207.9
Non-Polymers*	Number of molecules	4
	Total formula weight	817.4
All*	Total formula weight	240025.4

*Water molecules are not included.