3HTX
Crystal structure of small RNA methyltransferase HEN1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-11-17 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 96.995, 124.347, 101.376 |
| Unit cell angles | 90.00, 93.47, 90.00 |
Refinement procedure
| Resolution | 20.000 - 3.100 |
| R-factor | 0.26 |
| Rwork | 0.260 |
| R-free | 0.28800 |
| Structure solution method | MAD |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 3.210 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Rmerge | 0.097 | 0.489 |
| Number of reflections | 40959 | |
| <I/σ(I)> | 8.7 | 1.8 |
| Completeness [%] | 92.8 | 81.8 |
| Redundancy | 3 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.8 | 293 | 0.1 M Phosphate-citrate pH 4.8, 15% PEG 3350, 0.2 M NaCl, 0.01M NaBr, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.8 | 293 | 0.1 M Phosphate-citrate pH 4.8, 15% PEG 3350, 0.2 M NaCl, 0.01M NaBr, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | Phosphate-citrate | ||
| 2 | 1 | 1 | PEG 3350 | ||
| 3 | 1 | 1 | NaCl | ||
| 4 | 1 | 1 | NaBr | ||
| 5 | 1 | 2 | Phosphate-citrate | ||
| 6 | 1 | 2 | PEG 3350 | ||
| 7 | 1 | 2 | NaCl | ||
| 8 | 1 | 2 | NaBr |
