3HAX

Crystal structure of a substrate-bound Gar1-minus H/ACA RNP from Pyrococcus furiosus

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Probable tRNA pseudouridine synthase B	polymer	346	39453.9	1	UniProt (Q7LWY0) Pfam (PF08068) Pfam (PF01509) Pfam (PF16198) Pfam (PF01472)	Pyrococcus furiosus	Cbf5, tRNA pseudouridine 55 synthase, Psi55 synthase, tRNA-uridine isomerase, tRNA pseudouridylate synthase
2	B (C)	Ribosome biogenesis protein Nop10	polymer	60	7214.6	1	UniProt (Q8U1R4) Pfam (PF04135)	Pyrococcus furiosus
3	C (D)	50S ribosomal protein L7Ae	polymer	130	14314.6	1	UniProt (Q8U160) Pfam (PF01248)	Pyrococcus furiosus
4	D (E)	H/ACA RNA	polymer	63	20386.1	1
5	E (F)	5'-R(*AP*UP*AP*AP*UP*UP*(FHU)P*GP*AP*CP*UP*CP*AP*A)-3'	polymer	14	4452.7	1
6	F, G (A)	TRIETHYLENE GLYCOL	non-polymer		150.2	2	Chemie (PGE)
7	H, K (A, D)	1,2-ETHANEDIOL	non-polymer		62.1	2	Chemie (EDO)
8	I (C)	ZINC ION	non-polymer		65.4	1	Chemie (ZN)
9	J (C)	TETRAETHYLENE GLYCOL	non-polymer		194.2	1	Chemie (PG4)
10	L, M, N, O, P (E)	MAGNESIUM ION	non-polymer		24.3	5	Chemie (MG)
11	Q, R, S, T, U (A, C, D, E, F)	water	water		18.0	383	Chemie (HOH)

Sequence modifications

A: 4 - 343 (UniProt: Q7LWY0)

PDB	External Database	Details
His 344	-	expression tag
His 345	-	expression tag
His 346	-	expression tag
His 347	-	expression tag
His 348	-	expression tag
His 349	-	expression tag

C: 1 - 60 (UniProt: Q8U1R4)

PDB	External Database	Details
Lys 2	Arg 2	engineered mutation

D: 3 - 124 (UniProt: Q8U160)

PDB	External Database	Details
Met 1	-	expression tag
Ala 2	-	expression tag
His 125	-	expression tag
His 126	-	expression tag
His 127	-	expression tag
His 128	-	expression tag
His 129	-	expression tag
His 130	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	85821.9
Non-Polymers*	Number of molecules	11
	Total formula weight	805.6
All*	Total formula weight	86627.6

*Water molecules are not included.