3HAX
Crystal structure of a substrate-bound Gar1-minus H/ACA RNP from Pyrococcus furiosus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Collection date | 2008-05-29 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 187.809, 63.696, 83.848 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.110 |
| R-factor | 0.20389 |
| Rwork | 0.203 |
| R-free | 0.22034 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2hvy |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.959 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.160 |
| High resolution limit [Å] | 2.090 | 2.090 |
| Rmerge | 0.114 | 0.515 |
| Number of reflections | 57734 | |
| <I/σ(I)> | 13.7 | 2.7 |
| Completeness [%] | 99.6 | 99 |
| Redundancy | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 0.2M ammonium acetate, 0.15M magnesium acetate, 4% PEG 8000, 50mM cacodylate sodium, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
