3G4G

Crystal structure of human phosphodiesterase 4d with regulatory domain and d155871

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	cAMP-specific 3',5'-cyclic phosphodiesterase 4D	polymer	421	48370.8	4	UniProt (Q08499) Pfam (PF18100) Pfam (PF00233)	Homo sapiens (human)	DPDE3, PDE43
2	E, I, M, Q (A, B, C, D)	ZINC ION	non-polymer		65.4	4	Chemie (ZN)
3	F, J, N, R (A, B, C, D)	MAGNESIUM ION	non-polymer		24.3	4	Chemie (MG)
4	G, K, O, S (A, B, C, D)	CALCIUM ION	non-polymer		40.1	4	Chemie (CA)
5	H, L, P, T (A, B, C, D)	1-(3-nitrophenyl)-3-(pyridin-4-ylmethyl)pyrido[2,3-d]pyrimidine-2,4(1H,3H)-dione	non-polymer		375.3	4	Chemie (D71)
6	U, V, W, X (A, B, C, D)	water	water		18.0	167	Chemie (HOH)

Sequence modifications

A, B, C, D: 163 - 214 (UniProt: Q08499)

PDB	External Database	Details
Met 162	-	expression tag
Ala 579	-	expression tag
His 580	-	expression tag
His 581	-	expression tag
His 582	-	expression tag
His 583	-	expression tag
His 584	-	expression tag
His 585	-	expression tag

A, B, C, D: 224 - 578 (UniProt: Q08499)

PDB	External Database	Details
Lys 215	-	linker
Val 216	-	linker
Thr 217	-	linker
Ala 218	-	linker
Glu 219	-	linker
Glu 220	-	linker
Ala 221	-	linker
Pro 222	-	linker
Gln 223	-	linker

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	193483.2
Non-Polymers*	Number of molecules	16
	Total formula weight	2020.5
All*	Total formula weight	195503.7

*Water molecules are not included.