3G38

The catalytically inactive mutant Mth0212 (D151N) in complex with an 8 bp dsDNA

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Exodeoxyribonuclease	polymer	265	31429.7	1	UniProt (O26314) Pfam (PF03372)	Methanothermobacter thermautotrophicus	Mth0212
2	B (G)	5'-D(*CP*CP*TP*GP*UP*GP*CP*GP*AP*T)-3'	polymer	10	3022.0	1
3	C (K)	5'-D(*CP*GP*CP*GP*CP*AP*GP*GP*C)-3'	polymer	9	2741.8	1
4	D, E, F, G, H... (A)	GLYCEROL	non-polymer		92.1	6	Chemie (GOL)
5	J, K, L (A, G, K)	water	water		18.0	48	Chemie (HOH)

Sequence modifications

A: 1 - 257 (UniProt: O26314)

PDB	External Database	Details
Ala 2	Thr 2	engineered mutation
Asn 151	Asp 151	engineered mutation
Leu 258	-	expression tag
Glu 259	-	expression tag
His 260	-	expression tag
His 261	-	expression tag
His 262	-	expression tag
His 263	-	expression tag
His 264	-	expression tag
His 265	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	37193.4
Non-Polymers*	Number of molecules	6
	Total formula weight	552.6
All*	Total formula weight	37746.0

*Water molecules are not included.