3FYP
Crystal structure of the quadruple mutant (N23C/C246S/D247E/P249A) of 3-deoxy-D-manno-octulosonate 8-phosphate synthase (KDO8PS) from Neisseria meningitidis
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, C, D (A, B, C, D) | 3-deoxy-D-manno-octulosonic acid 8-phosphate synthetase | polymer | 280 | 30480.3 | 4 | UniProt (Q9JZ55) Pfam (PF00793) | Neisseria meningitidis serogroup B | 2-dehydro-3-deoxyphosphooctonate aldolase, Phospho-2-dehydro-3-deoxyoctonate aldolase, KDO-8-phosphate synthetase, KDO 8-P synthase, KDOPS |
| 2 | E, J (A, D) | CHLORIDE ION | non-polymer | 35.5 | 2 | Chemie (CL) | |||
| 3 | F, G (B) | GLYCEROL | non-polymer | 92.1 | 2 | Chemie (GOL) | |||
| 4 | H, K (C, D) | SODIUM ION | non-polymer | 23.0 | 2 | Chemie (NA) | |||
| 5 | I (D) | PHOSPHOENOLPYRUVATE | non-polymer | 168.0 | 1 | Chemie (PEP) | |||
| 6 | L (D) | MANGANESE (II) ION | non-polymer | 54.9 | 1 | Chemie (MN) | |||
| 7 | M, N, O, P (A, B, C, D) | water | water | 18.0 | 590 | Chemie (HOH) |
Sequence modifications
A, B, C, D: 1 - 280 (UniProt: Q9JZ55)
| PDB | External Database | Details |
|---|---|---|
| Cys 23 | Asn 23 | engineered mutation |
| Ser 246 | Cys 246 | engineered mutation |
| Glu 247 | Asp 247 | engineered mutation |
| Ala 249 | Pro 249 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 121921.2 | |
| Non-Polymers* | Number of molecules | 8 |
| Total formula weight | 524.1 | |
| All* | Total formula weight | 122445.2 |
