3FOW

Plasmodium Purine Nucleoside Phosphorylase V66I-V73I-Y160F Mutant

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Uridine phosphorylase, putative	polymer	276	30514.1	2	UniProt (Q8I3X4) Pfam (PF01048) In PDB	Plasmodium falciparum
2	A, B	PHOSPHATE ION	non-polymer		95.0	4	Chemie (PO4)
3	A, B	1,4-DIDEOXY-4-AZA-1-(S)-(9-DEAZAHYPOXANTHIN-9-YL)-D-RIBITOL	non-polymer		266.3	2	Chemie (IMH)
4		water	water		18.0	77	Chemie (HOH)

Sequence modifications

A, B: 2 - 245 (UniProt: Q8I3X4)

PDB	External Database	Details
Met -1	-	expression tag
Ala 0	-	expression tag
Leu 1	-	expression tag
Ile 66	Val 66	engineered mutation
Ile 73	Val 73	engineered mutation
Phe 160	Tyr 160	engineered mutation
Lys 246	-	expression tag
Gly 247	-	expression tag
Glu 248	-	expression tag
Phe 249	-	expression tag
Glu 250	-	expression tag
Ala 251	-	expression tag
Tyr 252	-	expression tag
Val 253	-	expression tag
Glu 254	-	expression tag
Gln 255	-	expression tag
Lys 256	-	expression tag
Leu 257	-	expression tag
Ile 258	-	expression tag
Ser 259	-	expression tag
Glu 260	-	expression tag
Glu 261	-	expression tag
Asp 262	-	expression tag
Leu 263	-	expression tag
Asn 264	-	expression tag
Ser 265	-	expression tag
Ala 266	-	expression tag
Val 267	-	expression tag
Asp 268	-	expression tag
His 269	-	expression tag
His 270	-	expression tag
His 271	-	expression tag
His 272	-	expression tag
His 273	-	expression tag
His 274	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	61028.2
Non-Polymers*	Number of molecules	6
	Total formula weight	912.4
All*	Total formula weight	61940.6

*Water molecules are not included.