3FOW
Plasmodium Purine Nucleoside Phosphorylase V66I-V73I-Y160F Mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2008-01-01 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | I 41 3 2 |
| Unit cell lengths | 234.969, 234.969, 234.969 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.420 - 2.800 |
| R-factor | 0.167 |
| Rwork | 0.165 |
| R-free | 0.20200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1nw4 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.500 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.3.0034) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.117 | 0.570 |
| Number of reflections | 27430 | |
| <I/σ(I)> | 3.3 | |
| Redundancy | 13.3 | 7.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 292.15 | 0.2 M Magnesium chloride, 0.1 M HEPES sodium pH 7.5, 30% v/v 2-Propanol, VAPOR DIFFUSION, SITTING DROP, temperature 292.15K |
