3FOW

Plasmodium Purine Nucleoside Phosphorylase V66I-V73I-Y160F Mutant

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0003824	molecular_function	catalytic activity
A	0004731	molecular_function	purine-nucleoside phosphorylase activity
A	0004850	molecular_function	uridine phosphorylase activity
A	0005829	cellular_component	cytosol
A	0006148	biological_process	inosine catabolic process
A	0006166	biological_process	purine ribonucleoside salvage
A	0006195	biological_process	purine nucleotide catabolic process
A	0006218	biological_process	uridine catabolic process
A	0009116	biological_process	nucleoside metabolic process
A	0016740	molecular_function	transferase activity
A	0016757	molecular_function	glycosyltransferase activity
A	0017061	molecular_function	S-methyl-5-thioadenosine phosphorylase activity
A	0047975	molecular_function	guanosine phosphorylase activity
B	0003824	molecular_function	catalytic activity
B	0004731	molecular_function	purine-nucleoside phosphorylase activity
B	0004850	molecular_function	uridine phosphorylase activity
B	0005829	cellular_component	cytosol
B	0006148	biological_process	inosine catabolic process
B	0006166	biological_process	purine ribonucleoside salvage
B	0006195	biological_process	purine nucleotide catabolic process
B	0006218	biological_process	uridine catabolic process
B	0009116	biological_process	nucleoside metabolic process
B	0016740	molecular_function	transferase activity
B	0016757	molecular_function	glycosyltransferase activity
B	0017061	molecular_function	S-methyl-5-thioadenosine phosphorylase activity
B	0047975	molecular_function	guanosine phosphorylase activity

Functional Information from PDB Data

site_id	AC1
Number of Residues	5
Details	BINDING SITE FOR RESIDUE PO4 A 275

Chain	Residue
A	ASP24
A	ARG27
A	SER91
A	IMH278
B	ARG45

---

site_id	AC2
Number of Residues	1
Details	BINDING SITE FOR RESIDUE PO4 A 276

Chain	Residue
B	ARG169

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site_id	AC3
Number of Residues	2
Details	BINDING SITE FOR RESIDUE PO4 A 277

Chain	Residue
A	ARG169
B	ARG116

---

site_id	AC4
Number of Residues	15
Details	BINDING SITE FOR RESIDUE IMH A 278

Chain	Residue
A	ARG88
A	SER91
A	CYS92
A	GLY93
A	PHE160
A	VAL181
A	GLU182
A	MET183
A	GLU184
A	ASP206
A	PRO209
A	PO4275
A	HOH307
B	HIS7
A	ILE66

---

site_id	AC5
Number of Residues	7
Details	BINDING SITE FOR RESIDUE PO4 B 275

Chain	Residue
A	ARG45
B	GLY23
B	ASP24
B	ARG27
B	GLY90
B	SER91
B	IMH276

---

site_id	AC6
Number of Residues	12
Details	BINDING SITE FOR RESIDUE IMH B 276

Chain	Residue
A	HIS7
B	ARG88
B	SER91
B	CYS92
B	GLY93
B	PHE160
B	GLU182
B	MET183
B	GLU184
B	ASP206
B	PO4275
B	HOH298

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	2
Details	Active site: {"description":"Proton donor","evidences":[{"source":"PubMed","id":"24416224","evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	2
Details	Binding site: {"evidences":[{"source":"PubMed","id":"24416224","evidenceCode":"ECO:0000305"},{"source":"Reference","evidenceCode":"ECO:0000305","citation":{"citationType":"submission","publicationDate":"NOV-2010","submissionDatabase":"PDB data bank","title":"Crystal Structure of Plasmodium falciparum purine nucleoside phosphorylase in complex with DADMe-ImmG.","authors":["Ho M.","Edwards A.A.","Almo S.C.","Schramm V.L."]}},{"source":"PDB","id":"3FOW","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3PHC","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	8
Details	Binding site: {"description":"in other chain","evidences":[{"source":"PubMed","id":"24416224","evidenceCode":"ECO:0000269"},{"source":"Reference","evidenceCode":"ECO:0000269","citation":{"citationType":"submission","publicationDate":"NOV-2010","submissionDatabase":"PDB data bank","title":"Crystal Structure of Plasmodium falciparum purine nucleoside phosphorylase in complex with DADMe-ImmG.","authors":["Ho M.","Edwards A.A.","Almo S.C.","Schramm V.L."]}},{"source":"PubMed","id":"14982926","evidenceCode":"ECO:0000305"},{"source":"PubMed","id":"19575810","evidenceCode":"ECO:0000305"},{"source":"PDB","id":"1NW4","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1Q1G","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3ENZ","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3FOW","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3PHC","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI4
Number of Residues	2
Details	Binding site: {"evidences":[{"source":"PubMed","id":"24416224","evidenceCode":"ECO:0000269"},{"source":"Reference","evidenceCode":"ECO:0000269","citation":{"citationType":"submission","publicationDate":"NOV-2010","submissionDatabase":"PDB data bank","title":"Crystal Structure of Plasmodium falciparum purine nucleoside phosphorylase in complex with DADMe-ImmG.","authors":["Ho M.","Edwards A.A.","Almo S.C.","Schramm V.L."]}},{"source":"PubMed","id":"14982926","evidenceCode":"ECO:0000305"},{"source":"PubMed","id":"19575810","evidenceCode":"ECO:0000305"},{"source":"PDB","id":"1NW4","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1Q1G","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3ENZ","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3FOW","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3PHC","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI5
Number of Residues	6
Details	Binding site: {"description":"in other chain","evidences":[{"source":"PubMed","id":"24416224","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"29440412","evidenceCode":"ECO:0000269"},{"source":"Reference","evidenceCode":"ECO:0000269","citation":{"citationType":"submission","publicationDate":"NOV-2010","submissionDatabase":"PDB data bank","title":"Crystal Structure of Plasmodium falciparum purine nucleoside phosphorylase in complex with DADMe-ImmG.","authors":["Ho M.","Edwards A.A.","Almo S.C.","Schramm V.L."]}},{"source":"PubMed","id":"14982926","evidenceCode":"ECO:0000305"},{"source":"PubMed","id":"19575810","evidenceCode":"ECO:0000305"},{"source":"PDB","id":"1NW4","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1Q1G","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3ENZ","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3FOW","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3PHC","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"6AQS","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"6AQU","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI6
Number of Residues	2
Details	Binding site: {"description":"in other chain","evidences":[{"source":"PubMed","id":"16131758","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"24416224","evidenceCode":"ECO:0000305"},{"source":"Reference","evidenceCode":"ECO:0000305","citation":{"citationType":"submission","publicationDate":"NOV-2010","submissionDatabase":"PDB data bank","title":"Crystal Structure of Plasmodium falciparum purine nucleoside phosphorylase in complex with DADMe-ImmG.","authors":["Ho M.","Edwards A.A.","Almo S.C.","Schramm V.L."]}},{"source":"PDB","id":"2BSX","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3FOW","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3PHC","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

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Catalytic Information from CSA

site_id	MCSA1
Number of Residues	4
Details	M-CSA 695

Chain	Residue	Details
A	ARG27	electrostatic stabiliser
A	ARG45	electrostatic stabiliser
A	ARG88	electrostatic stabiliser
A	ASP206	proton acceptor, proton donor

---

site_id	MCSA2
Number of Residues	4
Details	M-CSA 695

Chain	Residue	Details
B	ARG27	electrostatic stabiliser
B	ARG45	electrostatic stabiliser
B	ARG88	electrostatic stabiliser
B	ASP206	proton acceptor, proton donor

---