3FN1

E2-RING expansion of the NEDD8 cascade confers specificity to cullin modification.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	NEDD8-activating enzyme E1 catalytic subunit	polymer	98	10857.0	1	UniProt (Q8TBC4) Pfam (PF08825) In PDB	Homo sapiens (human)	Ubiquitin-like modifier-activating enzyme 3, Ubiquitin-activating enzyme 3, NEDD8-activating enzyme E1C, Ubiquitin-activating enzyme E1C
2	B	NEDD8-conjugating enzyme UBE2F	polymer	167	19104.6	1	UniProt (Q969M7) Pfam (PF00179) In PDB	Homo sapiens (human)	Ubiquitin-conjugating enzyme E2 F, NEDD8 protein ligase UBE2F, NEDD8 carrier protein UBE2F, NEDD8-conjugating enzyme 2
3		water	water		18.0	135	Chemie (HOH)

Sequence modifications

A: 347 - 442 (UniProt: Q8TBC4)

PDB	External Database	Details
Gly 345	-	expression tag
Ser 346	-	expression tag
Mse 394	Leu 415	engineered mutation

B: 27 - 185 (UniProt: Q969M7)

PDB	External Database	Details
Gly 19	-	expression tag
Ser 20	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	29961.6
All*	Total formula weight	29961.6

*Water molecules are not included.