3ER9
Crystal structure of the heterodimeric vaccinia virus mRNA polyadenylate polymerase complex with UU and 3'-deoxy ATP
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Cap-specific mRNA (nucleoside-2'-O-)-methyltransferase | polymer | 297 | 34589.0 | 1 | UniProt (P07617) Pfam (PF01358) | vaccinia virus WR | Poly(A) polymerase regulatory subunit, Poly(A) polymerase small subunit, PAP-S, VP39 |
| 2 | B (B) | Poly(A) polymerase catalytic subunit | polymer | 479 | 55574.1 | 1 | UniProt (P23371) Pfam (PF12630) Pfam (PF03296) Pfam (PF12629) | vaccinia virus WR | Poly(A) polymerase large subunit, PAP-L, VP55 |
| 3 | C (D) | 5'-R(UP*U)-3' | polymer | 2 | 567.4 | 1 | |||
| 4 | D, E, F (A) | GLYCEROL | non-polymer | 92.1 | 3 | Chemie (GOL) | |||
| 5 | G (B) | 3'-DEOXYADENOSINE-5'-TRIPHOSPHATE | non-polymer | 491.2 | 1 | Chemie (3AT) | |||
| 6 | H, I (B) | CALCIUM ION | non-polymer | 40.1 | 2 | Chemie (CA) | |||
| 7 | J, K (A, B) | water | water | 18.0 | 317 | Chemie (HOH) |
Sequence modifications
A: 1 - 297 (UniProt: P07617)
B: 1 - 479 (UniProt: P23371)
| PDB | External Database | Details |
|---|---|---|
| Ala 140 | Arg 140 | engineered mutation |
| Ala 142 | Lys 142 | engineered mutation |
| Ala 143 | Arg 143 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Ser 36 | Leu 36 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 3 |
| Total formula weight | 90730.5 | |
| Non-Polymers* | Number of molecules | 6 |
| Total formula weight | 847.6 | |
| All* | Total formula weight | 91578.1 |
