3ER9

Crystal structure of the heterodimeric vaccinia virus mRNA polyadenylate polymerase complex with UU and 3'-deoxy ATP

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Cap-specific mRNA (nucleoside-2'-O-)-methyltransferase	polymer	297	34589.0	1	UniProt (P07617) Pfam (PF01358) In PDB	vaccinia virus WR	Poly(A) polymerase regulatory subunit, Poly(A) polymerase small subunit, PAP-S, VP39
2	B	Poly(A) polymerase catalytic subunit	polymer	479	55574.1	1	UniProt (P23371) Pfam (PF12630) Pfam (PF03296) Pfam (PF12629) In PDB	vaccinia virus WR	Poly(A) polymerase large subunit, PAP-L, VP55
3	D	5'-R(UP*U)-3'	polymer	2	567.4	1
4	A	GLYCEROL	non-polymer		92.1	3	Chemie (GOL)
5	B	3'-DEOXYADENOSINE-5'-TRIPHOSPHATE	non-polymer		491.2	1	Chemie (3AT)
6	B	CALCIUM ION	non-polymer		40.1	2	Chemie (CA)
7		water	water		18.0	317	Chemie (HOH)

Sequence modifications

A: 1 - 297 (UniProt: P07617)

PDB	External Database	Details
Ala 140	Arg 140	engineered mutation
Ala 142	Lys 142	engineered mutation
Ala 143	Arg 143	engineered mutation

B: 1 - 479 (UniProt: P23371)

PDB	External Database	Details
Ser 36	Leu 36	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	90730.5
Non-Polymers*	Number of molecules	6
	Total formula weight	847.6
All*	Total formula weight	91578.1

*Water molecules are not included.