3ER9
Crystal structure of the heterodimeric vaccinia virus mRNA polyadenylate polymerase complex with UU and 3'-deoxy ATP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-2 |
Synchrotron site | SSRL |
Beamline | BL9-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-06-21 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.0000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 69.531, 91.977, 133.734 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 42.690 - 2.060 |
R-factor | 0.208 |
Rwork | 0.208 |
R-free | 0.25000 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 1.300 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Refinement software | CNS (1.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.190 |
High resolution limit [Å] | 2.060 | 2.060 |
Rmerge | 0.530 | 0.371 |
Number of reflections | 52976 | |
<I/σ(I)> | 27.3 | 3.04 |
Completeness [%] | 98.0 | 94.8 |
Redundancy | 4.2 | 3.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.7 | 295 | proteins(4-5mg.ml) in 10mM tris-HCl, pH 8.7, 75 mM NaCl, 0.5 mM DTT, mixed with equal volume buffer which composed of 10mM Tris-HCl, pH 8.7, 15-20% PEG 4000, 5% glycerol, 0.5 mM DTT. Room temperature for several days., VAPOR DIFFUSION, SITTING DROP, temperature 295K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | Tris-HCl | ||
2 | 1 | 1 | NaCl | ||
3 | 1 | 1 | DTT | ||
4 | 1 | 1 | PEG 4000 | ||
5 | 1 | 1 | glycerol | ||
6 | 1 | 2 | Tris-HCl | ||
7 | 1 | 2 | DTT | ||
8 | 1 | 2 | PEG 4000 | ||
9 | 1 | 2 | glycerol |