3ER8

Crystal structure of the heterodimeric vaccinia virus mRNA polyadenylate polymerase complex with two fragments of RNA

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Cap-specific mRNA (nucleoside-2'-O-)-methyltransferase	polymer	297	34589.0	2	UniProt (P07617) Pfam (PF01358) In PDB	vaccinia virus WR	Poly(A) polymerase regulatory subunit, Poly(A) polymerase small subunit, PAP-S, VP39
2	C, D	Poly(A) polymerase catalytic subunit	polymer	479	55574.1	2	UniProt (P23371) Pfam (PF12630) Pfam (PF03296) Pfam (PF12629) In PDB	vaccinia virus WR	Poly(A) polymerase large subunit, PAP-L, VP55
3	E	RNA/DNA chimera 5'-D(CP*CP*)R(UP*UP*)D(C)-3'	polymer	5	1434.9	1
4	F	RNA/DNA chimera 5'-R(P*UP*UP*)D(C)-3'	polymer	3	856.6	1
5	G, H	RNA/DNA chimera 5'-D(P*CP*)R(UP*U)-3'	polymer	3	856.6	2

Sequence modifications

A, B: 1 - 297 (UniProt: P07617)

PDB	External Database	Details
Ala 140	Arg 140	engineered mutation
Ala 142	Lys 142	engineered mutation
Ala 143	Arg 143	engineered mutation

C, D: 1 - 479 (UniProt: P23371)

PDB	External Database	Details
Ser 36	Leu 36	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	184330.8
All*	Total formula weight	184330.8

*Water molecules are not included.