3ER8
Crystal structure of the heterodimeric vaccinia virus mRNA polyadenylate polymerase complex with two fragments of RNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-12-14 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 1 |
| Unit cell lengths | 70.605, 77.241, 106.851 |
| Unit cell angles | 74.88, 74.00, 63.69 |
Refinement procedure
| Resolution | 38.900 - 3.180 |
| R-factor | 0.243 |
| Rwork | 0.243 |
| R-free | 0.28500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.400 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.380 |
| High resolution limit [Å] | 3.180 | 3.180 |
| Rmerge | 0.084 | 0.526 |
| Number of reflections | 30650 | |
| <I/σ(I)> | 14.5 | 2 |
| Completeness [%] | 93.1 | 87.2 |
| Redundancy | 3.7 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.7 | 295 | proteins(4-5mg.ml) in 10mM Tris-HCl, pH8.7, 75 mM NaCl, 0.5 mM DTT, mixed with equal volume buffer which composed of 10mM Tris-HCl, pH 8.7, 15-20% PEG 4000, 5% glycerol, 0.5 mM DTT. room temperature for several days., VAPOR DIFFUSION, SITTING DROP, temperature 295K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | Tris-HCl | ||
| 2 | 1 | 1 | NaCl | ||
| 3 | 1 | 1 | DTT | ||
| 4 | 1 | 1 | PEG 4000 | ||
| 5 | 1 | 1 | glycerol | ||
| 6 | 1 | 2 | Tris-HCl | ||
| 7 | 1 | 2 | DTT | ||
| 8 | 1 | 2 | PEG 4000 | ||
| 9 | 1 | 2 | glycerol |
