3E0V

Crystal structure of pyruvate kinase from Leishmania mexicana in complex with sulphate ions

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D, E...	Pyruvate kinase	polymer	539	58867.8	6	UniProt (Q27686) Pfam (PF00224) Pfam (PF02887) In PDB	Leishmania mexicana	PK
2	C, D, A, E, B...	SULFATE ION	non-polymer		96.1	40	Chemie (SO4)
3	A, E	GLYCEROL	non-polymer		92.1	3	Chemie (GOL)
4		water	water		18.0	314	Chemie (HOH)

Sequence modifications

A, B, C, D, E, F: 0 - 498 (UniProt: Q27686)

PDB	External Database	Details
Met -40	-	expression tag
Gly -39	-	expression tag
Ser -38	-	expression tag
Ser -37	-	expression tag
His -36	-	expression tag
His -35	-	expression tag
His -34	-	expression tag
His -33	-	expression tag
His -32	-	expression tag
His -31	-	expression tag
Ser -30	-	expression tag
Ser -29	-	expression tag
Gly -28	-	expression tag
Leu -27	-	expression tag
Val -26	-	expression tag
Pro -25	-	expression tag
Arg -24	-	expression tag
Gly -23	-	expression tag
Ser -22	-	expression tag
His -21	-	expression tag
Met -20	-	expression tag
Gly -19	-	expression tag
Ser -18	-	expression tag
Ser -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
Ser -10	-	expression tag
Ser -9	-	expression tag
Gly -8	-	expression tag
Leu -7	-	expression tag
Val -6	-	expression tag
Pro -5	-	expression tag
Arg -4	-	expression tag
Gly -3	-	expression tag
Ser -2	-	expression tag
His -1	-	expression tag
Ser 382	Gly 383	conflict
Tyr 389	Ser 390	conflict
Arg 404	Ala 405	conflict
Ser 405	Gly 406	conflict
Trp 451	Glu 452	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	353206.6
Non-Polymers*	Number of molecules	43
	Total formula weight	4118.8
All*	Total formula weight	357325.4

*Water molecules are not included.