3D70

Crystal structure of E253A mutant of BMRR bound to 22-bp oligonucleotide

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	BMR promoter DNA	polymer	284	33420.2	1	UniProt (P39075) Pfam (PF00376) Pfam (PF06445) In PDB	synthetic construct
2	B	Multidrug-efflux transporter 1 regulator	polymer	24	7362.7	1		Bacillus subtilis
3	A	IMIDAZOLE	non-polymer		69.1	6	Chemie (IMD)
4	A	GLYCEROL	non-polymer		92.1	3	Chemie (GOL)
5		water	water		18.0	49	Chemie (HOH)

Sequence modifications

A: 1 - 278 (UniProt: P39075)

PDB	External Database	Details
Ala 253	Glu 253	engineered mutation
Leu 277	Ala 277	engineered mutation
Asp 278	Glu 278	engineered mutation
His 279	-	expression tag
His 280	-	expression tag
His 281	-	expression tag
His 282	-	expression tag
His 283	-	expression tag
His 284	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	40782.9
Non-Polymers*	Number of molecules	9
	Total formula weight	690.8
All*	Total formula weight	41473.7

*Water molecules are not included.