3D70
Crystal structure of E253A mutant of BMRR bound to 22-bp oligonucleotide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-1 |
| Synchrotron site | SSRL |
| Beamline | BL9-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-12-20 |
| Detector | ADSC QUANTUM 4 |
| Spacegroup name | P 43 2 2 |
| Unit cell lengths | 106.170, 106.170, 146.880 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 52.500 - 2.800 |
| R-factor | 0.234 |
| Rwork | 0.234 |
| R-free | 0.27300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1exj |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.400 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | EPMR |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 52.500 | 2.870 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.050 | 0.510 |
| Number of reflections | 20870 | |
| <I/σ(I)> | 12.2 | 3.7 |
| Completeness [%] | 98.5 | 100 |
| Redundancy | 5.1 | 5.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | SODIUM CITRATE, IMIDAZOLE, TRIFLUOROETHANOL, pH 8.00, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | SODIUM CITRATE | ||
| 2 | 1 | 2 | SODIUM CITRATE | ||
| 3 | 1 | 1 | IMIDAZOLE | ||
| 4 | 1 | 2 | IMIDAZOLE | ||
| 5 | 1 | 1 | TRIFLUOROETHANOL |
