3CV9
Crystal structure of vitamin D hydroxylase cytochrome P450 105A1 (R73A/R84A mutant) in complex with 1alpha,25-dihydroxyvitamin D3
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A (A) | Cytochrome P450-SU1 | polymer | 412 | 44905.6 | 1 | UniProt (P18326) Pfam (PF00067) | Streptomyces griseolus | P450-CVA1, CYP105A1 |
2 | B (A) | PROTOPORPHYRIN IX CONTAINING FE | non-polymer | 616.5 | 1 | Chemie (HEM) | |||
3 | C (A) | 5-{2-[1-(5-HYDROXY-1,5-DIMETHYL-HEXYL)-7A-METHYL-OCTAHYDRO-INDEN-4-YLIDENE]-ETHYLIDENE}-4-METHYLENE-CYCLOHEXANE-1,3-DIOL | non-polymer | 416.6 | 1 | Chemie (VDX) | |||
4 | D (A) | water | water | 18.0 | 463 | Chemie (HOH) |
Sequence modifications
A: 1 - 406 (UniProt: P18326)
PDB | External Database | Details |
---|---|---|
Ala 73 | Arg 73 | engineered mutation |
Ala 84 | Arg 84 | engineered mutation |
Gln 308 | His 308 | SEE REMARK 999 |
His 407 | - | expression tag |
His 408 | - | expression tag |
His 409 | - | expression tag |
His 410 | - | expression tag |
His 411 | - | expression tag |
His 412 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 1 |
Total formula weight | 44905.6 | |
Non-Polymers* | Number of molecules | 2 |
Total formula weight | 1033.1 | |
All* | Total formula weight | 45938.8 |