3AY4

Crystal structure of nonfucosylated Fc complexed with bis-glycosylated soluble form of Fc gamma receptor IIIa

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Ig gamma-1 chain C region	polymer	223	25097.4	2	UniProt (P01857) Pfam (PF07654)	Homo sapiens (human)	IGG1
2	C (C)	Low affinity immunoglobulin gamma Fc region receptor III-A	polymer	179	20641.0	1	UniProt (P08637) Pfam (PF13895)	Homo sapiens (human)
3	D, E (D, E)	beta-D-galactopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-2)-alpha-D-mannopyranose-(1-6)-[2-acetamido-2-deoxy-beta-D-glucopyranose-(1-2)-alpha-D-mannopyranose-(1-3)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		1479.3	2	In PDB GlyTouCan (G89993FE)
4	F (F)	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[2-acetamido-2-deoxy-beta-D-glucopyranose-(1-2)-alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-[alpha-L-fucopyranose-(1-6)]2-acetamido-2-deoxy-beta-D-glucopyranose	branched		1463.3	1	In PDB GlyTouCan (G80858MF)
5	G (G)	beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		586.5	1	In PDB GlyTouCan (G15407YE)
6	H, I, J (A, B, C)	water	water		18.0	307	Chemie (HOH)

Sequence modifications

C: 3 - 175 (UniProt: P08637)

PDB	External Database	Details
Gln 38	Asn 56	engineered mutation
Gln 74	Asn 92	engineered mutation
Val 158	Phe 176	SEE REMARK 999
Gln 169	Asn 187	engineered mutation
His 176	-	expression tag
His 177	-	expression tag
His 178	-	expression tag
His 179	-	expression tag
His 180	-	expression tag
His 181	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	70835.9
Branched	Number of molecules	4
	Total formula weight	5008.6
All*	Total formula weight	75844.5

*Water molecules are not included.