3AY4
Crystal structure of nonfucosylated Fc complexed with bis-glycosylated soluble form of Fc gamma receptor IIIa
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL44XU |
Synchrotron site | SPring-8 |
Beamline | BL44XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-12-07 |
Detector | RAYONIX MX225HE |
Wavelength(s) | 0.9 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 77.262, 77.262, 350.343 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 43.790 - 2.200 |
R-factor | 0.225 |
Rwork | 0.223 |
R-free | 0.26285 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1e4k |
RMSD bond length | 0.007 |
RMSD bond angle | 1.033 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 87.600 | 2.240 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.089 | 0.372 |
Number of reflections | 53300 | |
<I/σ(I)> | 20.5 | |
Completeness [%] | 96.4 | 77.4 |
Redundancy | 12.6 | 6.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 12% PEG 20000, 0.1M MES, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |