3AWI

Bifunctional tRNA modification enzyme MnmC from Escherichia coli

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D, E...	tRNA 5-methylaminomethyl-2-thiouridine biosynthesis bifunctional protein mnmC	polymer	688	76679.3	6	UniProt (P77182) Pfam (PF05430) Pfam (PF01266) In PDB	Escherichia coli	tRNA mnm(5)s(2)U biosynthesis bifunctional protein, tRNA (mnm(5)s(2)U34)-methyltransferase, FAD-dependent cmnm(5)s(2)U34 oxidoreductase
2	A, B, C, D, E...	FLAVIN-ADENINE DINUCLEOTIDE	non-polymer		785.5	6	Chemie (FAD)
3	A, B, C, D, E...	SULFATE ION	non-polymer		96.1	11	Chemie (SO4)
4		water	water		18.0	61	Chemie (HOH)

Sequence modifications

A, B, C, D, E, F: 1 - 668 (UniProt: P77182)

PDB	External Database	Details
Met -19	-	expression tag
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	460075.6
Non-Polymers*	Number of molecules	17
	Total formula weight	5770.0
All*	Total formula weight	465845.6

*Water molecules are not included.