Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

3AWI

Bifunctional tRNA modification enzyme MnmC from Escherichia coli

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0002097	biological_process	tRNA wobble base modification
A	0002098	biological_process	tRNA wobble uridine modification
A	0003824	molecular_function	catalytic activity
A	0004808	molecular_function	tRNA (5-methylaminomethyl-2-thiouridylate)(34)-methyltransferase activity
A	0005737	cellular_component	cytoplasm
A	0008033	biological_process	tRNA processing
A	0008168	molecular_function	methyltransferase activity
A	0008757	molecular_function	S-adenosylmethionine-dependent methyltransferase activity
A	0016491	molecular_function	oxidoreductase activity
A	0016645	molecular_function	oxidoreductase activity, acting on the CH-NH group of donors
A	0016740	molecular_function	transferase activity
A	0030488	biological_process	tRNA methylation
A	0032259	biological_process	methylation
A	0050660	molecular_function	flavin adenine dinucleotide binding
A	0071949	molecular_function	FAD binding
B	0002097	biological_process	tRNA wobble base modification
B	0002098	biological_process	tRNA wobble uridine modification
B	0003824	molecular_function	catalytic activity
B	0004808	molecular_function	tRNA (5-methylaminomethyl-2-thiouridylate)(34)-methyltransferase activity
B	0005737	cellular_component	cytoplasm
B	0008033	biological_process	tRNA processing
B	0008168	molecular_function	methyltransferase activity
B	0008757	molecular_function	S-adenosylmethionine-dependent methyltransferase activity
B	0016491	molecular_function	oxidoreductase activity
B	0016645	molecular_function	oxidoreductase activity, acting on the CH-NH group of donors
B	0016740	molecular_function	transferase activity
B	0030488	biological_process	tRNA methylation
B	0032259	biological_process	methylation
B	0050660	molecular_function	flavin adenine dinucleotide binding
B	0071949	molecular_function	FAD binding
C	0002097	biological_process	tRNA wobble base modification
C	0002098	biological_process	tRNA wobble uridine modification
C	0003824	molecular_function	catalytic activity
C	0004808	molecular_function	tRNA (5-methylaminomethyl-2-thiouridylate)(34)-methyltransferase activity
C	0005737	cellular_component	cytoplasm
C	0008033	biological_process	tRNA processing
C	0008168	molecular_function	methyltransferase activity
C	0008757	molecular_function	S-adenosylmethionine-dependent methyltransferase activity
C	0016491	molecular_function	oxidoreductase activity
C	0016645	molecular_function	oxidoreductase activity, acting on the CH-NH group of donors
C	0016740	molecular_function	transferase activity
C	0030488	biological_process	tRNA methylation
C	0032259	biological_process	methylation
C	0050660	molecular_function	flavin adenine dinucleotide binding
C	0071949	molecular_function	FAD binding
D	0002097	biological_process	tRNA wobble base modification
D	0002098	biological_process	tRNA wobble uridine modification
D	0003824	molecular_function	catalytic activity
D	0004808	molecular_function	tRNA (5-methylaminomethyl-2-thiouridylate)(34)-methyltransferase activity
D	0005737	cellular_component	cytoplasm
D	0008033	biological_process	tRNA processing
D	0008168	molecular_function	methyltransferase activity
D	0008757	molecular_function	S-adenosylmethionine-dependent methyltransferase activity
D	0016491	molecular_function	oxidoreductase activity
D	0016645	molecular_function	oxidoreductase activity, acting on the CH-NH group of donors
D	0016740	molecular_function	transferase activity
D	0030488	biological_process	tRNA methylation
D	0032259	biological_process	methylation
D	0050660	molecular_function	flavin adenine dinucleotide binding
D	0071949	molecular_function	FAD binding
E	0002097	biological_process	tRNA wobble base modification
E	0002098	biological_process	tRNA wobble uridine modification
E	0003824	molecular_function	catalytic activity
E	0004808	molecular_function	tRNA (5-methylaminomethyl-2-thiouridylate)(34)-methyltransferase activity
E	0005737	cellular_component	cytoplasm
E	0008033	biological_process	tRNA processing
E	0008168	molecular_function	methyltransferase activity
E	0008757	molecular_function	S-adenosylmethionine-dependent methyltransferase activity
E	0016491	molecular_function	oxidoreductase activity
E	0016645	molecular_function	oxidoreductase activity, acting on the CH-NH group of donors
E	0016740	molecular_function	transferase activity
E	0030488	biological_process	tRNA methylation
E	0032259	biological_process	methylation
E	0050660	molecular_function	flavin adenine dinucleotide binding
E	0071949	molecular_function	FAD binding
F	0002097	biological_process	tRNA wobble base modification
F	0002098	biological_process	tRNA wobble uridine modification
F	0003824	molecular_function	catalytic activity
F	0004808	molecular_function	tRNA (5-methylaminomethyl-2-thiouridylate)(34)-methyltransferase activity
F	0005737	cellular_component	cytoplasm
F	0008033	biological_process	tRNA processing
F	0008168	molecular_function	methyltransferase activity
F	0008757	molecular_function	S-adenosylmethionine-dependent methyltransferase activity
F	0016491	molecular_function	oxidoreductase activity
F	0016645	molecular_function	oxidoreductase activity, acting on the CH-NH group of donors
F	0016740	molecular_function	transferase activity
F	0030488	biological_process	tRNA methylation
F	0032259	biological_process	methylation
F	0050660	molecular_function	flavin adenine dinucleotide binding
F	0071949	molecular_function	FAD binding

Functional Information from PDB Data

site_id	AC1
Number of Residues	31
Details	BINDING SITE FOR RESIDUE FAD A 901

Chain	Residue
A	ILE270
A	ASP296
A	GLY302
A	ALA303
A	SER304
A	ASN306
A	ALA310
A	TYR430
A	GLN431
A	LEU432
A	ASN461
A	GLY271
A	GLY462
A	GLN464
A	VAL483
A	TYR504
A	LEU615
A	GLY616
A	SER617
A	ARG618
A	GLY619
A	LEU620
A	GLY272
A	HOH673
A	HOH681
A	GLY273
A	ILE274
A	ALA275
A	TYR293
A	CYS294
A	ALA295

site_id	AC2
Number of Residues	4
Details	BINDING SITE FOR RESIDUE SO4 A 669

Chain	Residue
A	HIS573
A	GLY616
A	SER617
A	ARG618

site_id	AC3
Number of Residues	4
Details	BINDING SITE FOR RESIDUE SO4 A 670

Chain	Residue
A	CYS407
A	ALA409
A	ARG413
A	ARG563

site_id	AC4
Number of Residues	30
Details	BINDING SITE FOR RESIDUE FAD B 901

Chain	Residue
B	ILE270
B	GLY271
B	GLY272
B	GLY273
B	ILE274
B	ALA275
B	TYR293
B	CYS294
B	ALA295
B	ASP296
B	GLY302
B	ALA303
B	SER304
B	ASN306
B	ALA310
B	TYR430
B	GLN431
B	LEU432
B	ASN461
B	GLY462
B	GLN464
B	PHE468
B	VAL483
B	TYR504
B	LEU615
B	GLY616
B	SER617
B	ARG618
B	GLY619
B	LEU620

site_id	AC5
Number of Residues	4
Details	BINDING SITE FOR RESIDUE SO4 B 669

Chain	Residue
B	HIS573
B	GLY616
B	SER617
B	ARG618

site_id	AC6
Number of Residues	4
Details	BINDING SITE FOR RESIDUE SO4 B 670

Chain	Residue
B	ARG307
B	ALA409
B	ARG413
B	ARG563

site_id	AC7
Number of Residues	29
Details	BINDING SITE FOR RESIDUE FAD C 901

Chain	Residue
C	TYR504
C	LEU615
C	GLY616
C	SER617
C	ARG618
C	GLY619
C	LEU620
C	ILE270
C	GLY271
C	GLY272
C	GLY273
C	ILE274
C	ALA275
C	CYS294
C	ALA295
C	ASP296
C	GLY302
C	ALA303
C	SER304
C	ASN306
C	ALA310
C	TYR430
C	GLN431
C	LEU432
C	ASN461
C	GLY462
C	GLN464
C	PHE468
C	VAL483

site_id	AC8
Number of Residues	3
Details	BINDING SITE FOR RESIDUE SO4 C 669

Chain	Residue
C	HIS573
C	SER617
C	ARG618

site_id	AC9
Number of Residues	4
Details	BINDING SITE FOR RESIDUE SO4 C 670

Chain	Residue
C	CYS407
C	ALA409
C	ARG413
C	ARG563

site_id	BC1
Number of Residues	4
Details	BINDING SITE FOR RESIDUE SO4 C 671

Chain	Residue
B	ALA374
C	GLY214
C	ARG217
C	ARG218

site_id	BC2
Number of Residues	28
Details	BINDING SITE FOR RESIDUE FAD D 901

Chain	Residue
D	GLY271
D	GLY272
D	GLY273
D	ILE274
D	ALA275
D	CYS294
D	ALA295
D	ASP296
D	ALA303
D	SER304
D	ASN306
D	ALA310
D	TYR430
D	GLN431
D	LEU432
D	ASN461
D	GLY462
D	GLN464
D	GLY481
D	TYR504
D	GLY565
D	ARG567
D	LEU615
D	GLY616
D	SER617
D	ARG618
D	GLY619
D	LEU620

site_id	BC3
Number of Residues	4
Details	BINDING SITE FOR RESIDUE SO4 D 669

Chain	Residue
D	ARG307
D	CYS407
D	ARG413
D	ARG563

site_id	BC4
Number of Residues	4
Details	BINDING SITE FOR RESIDUE SO4 D 670

Chain	Residue
D	HIS573
D	GLY616
D	SER617
D	ARG618

site_id	BC5
Number of Residues	30
Details	BINDING SITE FOR RESIDUE FAD E 901

Chain	Residue
E	ILE270
E	GLY271
E	GLY272
E	GLY273
E	ILE274
E	ALA275
E	TYR293
E	CYS294
E	ALA295
E	ASP296
E	GLY302
E	ALA303
E	SER304
E	ASN306
E	ALA310
E	TYR430
E	GLN431
E	LEU432
E	ASN461
E	GLY462
E	GLN464
E	GLY481
E	TYR504
E	ARG567
E	LEU615
E	GLY616
E	SER617
E	ARG618
E	GLY619
E	LEU620

site_id	BC6
Number of Residues	4
Details	BINDING SITE FOR RESIDUE SO4 E 669

Chain	Residue
E	ARG307
E	CYS407
E	ARG413
E	ARG563

site_id	BC7
Number of Residues	31
Details	BINDING SITE FOR RESIDUE FAD F 901

Chain	Residue
F	ILE270
F	GLY271
F	GLY272
F	GLY273
F	ILE274
F	ALA275
F	CYS294
F	ALA295
F	ASP296
F	GLY302
F	ALA303
F	SER304
F	ASN306
F	ALA310
F	TYR430
F	LEU432
F	ASN461
F	GLY462
F	GLN464
F	ARG467
F	GLY481
F	GLN482
F	VAL483
F	TYR504
F	GLY565
F	ARG567
F	GLY616
F	SER617
F	ARG618
F	GLY619
F	LEU620

site_id	BC8
Number of Residues	5
Details	BINDING SITE FOR RESIDUE SO4 F 669

Chain	Residue
F	TYR521
F	HIS573
F	GLY616
F	SER617
F	ARG618

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)
		EDMap 2fo-fc (MTZ)