3AWI
Bifunctional tRNA modification enzyme MnmC from Escherichia coli
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-5A |
Synchrotron site | Photon Factory |
Beamline | BL-5A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-11-17 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 92.138, 243.022, 175.527 |
Unit cell angles | 90.00, 90.42, 90.00 |
Refinement procedure
Resolution | 46.068 - 3.000 |
R-factor | 0.205 |
Rwork | 0.204 |
R-free | 0.25140 |
Structure solution method | SAD |
RMSD bond length | 0.010 |
RMSD bond angle | 1.026 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.7_650)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.110 |
High resolution limit [Å] | 3.000 | 3.000 |
Number of reflections | 151613 | |
Completeness [%] | 98.3 | 94 |
Redundancy | 4.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 293 | 0.1 M Bis-Tris (pH 5.5), 25% PEG 3350, 0.25M ammonium sulfate, 10mM hexamine cobalt(III), VAPOR DIFFUSION, SITTING DROP, temperature 293K |