2YV0
Structural and Thermodynamic Analyses of E. coli ribonuclease HI Variant with Quintuple Thermostabilizing Mutations
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (X) | Ribonuclease HI | polymer | 155 | 17560.9 | 1 | UniProt (P0A7Y4) Pfam (PF00075) | Escherichia coli | RNase HI, Ribonuclease H, RNase H |
| 2 | B (X) | water | water | 18.0 | 217 | Chemie (HOH) |
Sequence modifications
X: 1 - 155 (UniProt: P0A7Y4)
| PDB | External Database | Details |
|---|---|---|
| Ala 23 | Gly 23 | engineered mutation |
| Pro 62 | His 62 | engineered mutation |
| Leu 74 | Val 74 | engineered mutation |
| Gly 95 | Lys 95 | engineered mutation |
| His 134 | Asp 134 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 1 |
| Total formula weight | 17560.9 | |
| All* | Total formula weight | 17560.9 |
