2YV0

Structural and Thermodynamic Analyses of E. coli ribonuclease HI Variant with Quintuple Thermostabilizing Mutations

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL38B1
Synchrotron site	SPring-8
Beamline	BL38B1
Collection date	2006-03-09
Wavelength(s)	0.80
Spacegroup name	P 21 21 21
Unit cell lengths	43.371, 86.483, 34.816
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.910 - 1.400
R-factor	0.18243
Rwork	0.182
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2rn2
RMSD bond length	0.025
RMSD bond angle	1.876
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	REFMAC (5.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.370
High resolution limit [Å]	1.320	1.320
Rmerge	0.032	0.134
Number of reflections	31520
<I/σ(I)>	62	12.1
Completeness [%]	99.8	99.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP			VAPOR DIFFUSION, SITTING DROP