2YV0

Structural and Thermodynamic Analyses of E. coli ribonuclease HI Variant with Quintuple Thermostabilizing Mutations

Functional Information from GO Data

Chain	GOid	namespace	contents
X	0000287	molecular_function	magnesium ion binding
X	0003676	molecular_function	nucleic acid binding
X	0004519	molecular_function	endonuclease activity
X	0004523	molecular_function	RNA-DNA hybrid ribonuclease activity
X	0005515	molecular_function	protein binding
X	0005737	cellular_component	cytoplasm
X	0006401	biological_process	RNA catabolic process
X	0043137	biological_process	DNA replication, removal of RNA primer
X	0046872	molecular_function	metal ion binding

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	4
Details	BINDING: BINDING => ECO:0000269|PubMed:1311386

Chain	Residue	Details
X	ASP10
X	GLU48
X	ASP70
X	HIS134

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Catalytic Information from CSA

site_id	MCSA1
Number of Residues	6
Details	M-CSA 163

Chain	Residue	Details
X	ASP10	metal ligand
X	GLY11	metal ligand
X	GLU48	increase acidity, increase basicity, unknown
X	ASP70	metal ligand
X	HIS124	hydrogen bond acceptor, hydrogen bond donor, proton acceptor, proton donor, unknown
X	HIS134	increase acidity, metal ligand, proton acceptor, proton donor

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