2XWB
Crystal Structure of Complement C3b in complex with Factors B and D
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, C (A, C) | COMPLEMENT C3B BETA CHAIN | polymer | 642 | 71154.0 | 2 | UniProt (P01024) Pfam (PF17790) Pfam (PF01835) Pfam (PF17791) Pfam (PF17789) Pfam (PF07703) | HOMO SAPIENS (HUMAN) | |
| 2 | B, D (B, D) | COMPLEMENT C3B ALPHA' CHAIN | polymer | 912 | 103759.8 | 2 | UniProt (P01024) Pfam (PF07677) Pfam (PF01759) Pfam (PF00207) Pfam (PF21406) Pfam (PF07678) Pfam (PF21308) | HOMO SAPIENS (HUMAN) | |
| 3 | E, F (F, H) | COMPLEMENT FACTOR B | polymer | 732 | 82156.7 | 2 | UniProt (P00751) Pfam (PF00084) Pfam (PF00092) Pfam (PF00089) | HOMO SAPIENS (HUMAN) | C3/C5 CONVERTASE, GLYCINE-RICH BETA GLYCOPROTEIN, GBG, PBF2, PROPERDIN FACTOR B |
| 4 | G, H (I, J) | COMPLEMENT FACTOR D | polymer | 228 | 24422.8 | 2 | UniProt (P00746) Pfam (PF00089) | HOMO SAPIENS (HUMAN) | |
| 5 | I, J, K (E, G, K) | 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 424.4 | 3 | In PDB GlyTouCan (G42666HT) | |||
| 6 | L, M (L, M) | beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 586.5 | 2 | In PDB GlyTouCan (G15407YE) | |||
| 7 | N, O, Q, T (A, C, F, H) | MAGNESIUM ION | non-polymer | 24.3 | 4 | Chemie (MG) | |||
| 8 | P, R, S, U, V (D, F, H) | 2-acetamido-2-deoxy-beta-D-glucopyranose | non-polymer | 221.2 | 5 | Chemie (NAG) | |||
| 9 | W, X (I, J) | GLYCEROL | non-polymer | 92.1 | 2 | Chemie (GOL) | |||
| 10 | Y, Z (F, H) | water | water | 18.0 | 4 | Chemie (HOH) |
Sequence modifications
B, D: 730 - 1641 (UniProt: P01024)
F, H: 10 - 739 (UniProt: P00751)
I, J: 1 - 228 (UniProt: P00746)
| PDB | External Database | Details |
|---|---|---|
| Glu 991 | Gln 1013 | SEE REMARK 999 |
| PDB | External Database | Details |
|---|---|---|
| Gly 254 | Asp 279 | engineered mutation |
| Asp 260 | Asn 285 | engineered mutation |
| Ala 740 | - | expression tag |
| Ala 741 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Ala 183 | Ser 208 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 8 |
| Total formula weight | 562986.6 | |
| Branched | Number of molecules | 5 |
| Total formula weight | 2446.3 | |
| Non-Polymers* | Number of molecules | 11 |
| Total formula weight | 1387.4 | |
| All* | Total formula weight | 566820.4 |
