2XWB
Crystal Structure of Complement C3b in complex with Factors B and D
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-06-12 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 |
| Unit cell lengths | 108.275, 135.775, 149.975 |
| Unit cell angles | 95.47, 110.69, 113.39 |
Refinement procedure
| Resolution | 65.950 - 3.490 |
| R-factor | 0.192 |
| Rwork | 0.189 |
| R-free | 0.24400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2a74 |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.488 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE: 1.5_2)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 67.420 | 3.690 |
| High resolution limit [Å] | 3.500 | 3.500 |
| Rmerge | 0.140 | 0.630 |
| Number of reflections | 88224 | |
| <I/σ(I)> | 8.8 | 2.2 |
| Completeness [%] | 99.2 | 99 |
| Redundancy | 3.3 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.7 | PH 7.7 |
