2XNS

Crystal Structure Of Human G alpha i1 Bound To A Designed Helical Peptide Derived From The Goloco Motif Of RGS14

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	GUANINE NUCLEOTIDE-BINDING PROTEIN G(I) SUBUNIT ALPHA-1	polymer	327	37374.6	2	UniProt (P63096) Pfam (PF00503)	HOMO SAPIENS (HUMAN)	ADENYLATE CYCLASE-INHIBITING G ALPHA PROTEIN
2	C, D (C, D)	REGULATOR OF G-PROTEIN SIGNALING 14	polymer	40	4695.2	2	UniProt (O43566) Pfam (PF02188)	HOMO SAPIENS	RGS14
3	E, G (A, B)	GUANOSINE-5'-DIPHOSPHATE	non-polymer		443.2	2	Chemie (GDP)
4	F, H (A, B)	S,R MESO-TARTARIC ACID	non-polymer		150.1	2	Chemie (SRT)
5	I, J (C, D)	SULFATE ION	non-polymer		96.1	2	Chemie (SO4)
6	K, L, M, N (A, B, C, D)	water	water		18.0	10	Chemie (HOH)

Sequence modifications

A, B: 30 - 354 (UniProt: P63096)

PDB	External Database	Details
Ser 28	-	expression tag
Asn 29	-	expression tag

C, D: 496 - 516 (UniProt: O43566)

PDB	External Database	Details
Gly 517	-	expression tag
Leu 518	-	expression tag
Leu 519	-	expression tag
Ser 520	-	expression tag
Asn 521	-	expression tag
Glu 522	-	expression tag
Glu 523	-	expression tag
Val 524	-	expression tag
Phe 525	-	expression tag
Arg 526	-	expression tag
Ala 527	-	expression tag
Leu 528	-	expression tag
Arg 529	-	expression tag
Asp 530	-	expression tag
Phe 531	-	expression tag
Asp 532	-	expression tag
Arg 533	-	expression tag
Trp 534	-	expression tag
Phe 535	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	84139.5
Non-Polymers*	Number of molecules	6
	Total formula weight	1378.7
All*	Total formula weight	85518.2

*Water molecules are not included.