Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

2XNS

Crystal Structure Of Human G alpha i1 Bound To A Designed Helical Peptide Derived From The Goloco Motif Of RGS14

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000166	molecular_function	nucleotide binding
A	0000287	molecular_function	magnesium ion binding
A	0001664	molecular_function	G protein-coupled receptor binding
A	0003924	molecular_function	GTPase activity
A	0003925	molecular_function	G protein activity
A	0005515	molecular_function	protein binding
A	0005525	molecular_function	GTP binding
A	0005634	cellular_component	nucleus
A	0005654	cellular_component	nucleoplasm
A	0005730	cellular_component	nucleolus
A	0005737	cellular_component	cytoplasm
A	0005765	cellular_component	lysosomal membrane
A	0005794	cellular_component	Golgi apparatus
A	0005813	cellular_component	centrosome
A	0005829	cellular_component	cytosol
A	0005834	cellular_component	heterotrimeric G-protein complex
A	0005856	cellular_component	cytoskeleton
A	0005886	cellular_component	plasma membrane
A	0005938	cellular_component	cell cortex
A	0007165	biological_process	signal transduction
A	0007186	biological_process	G protein-coupled receptor signaling pathway
A	0007188	biological_process	adenylate cyclase-modulating G protein-coupled receptor signaling pathway
A	0007189	biological_process	adenylate cyclase-activating G protein-coupled receptor signaling pathway
A	0007193	biological_process	adenylate cyclase-inhibiting G protein-coupled receptor signaling pathway
A	0007198	biological_process	adenylate cyclase-inhibiting serotonin receptor signaling pathway
A	0007218	biological_process	neuropeptide signaling pathway
A	0010854	molecular_function	adenylate cyclase regulator activity
A	0010855	molecular_function	adenylate cyclase inhibitor activity
A	0016020	cellular_component	membrane
A	0016787	molecular_function	hydrolase activity
A	0019001	molecular_function	guanyl nucleotide binding
A	0019003	molecular_function	GDP binding
A	0030496	cellular_component	midbody
A	0031683	molecular_function	G-protein beta/gamma-subunit complex binding
A	0031749	molecular_function	D2 dopamine receptor binding
A	0031821	molecular_function	G protein-coupled serotonin receptor binding
A	0034451	cellular_component	centriolar satellite
A	0034695	biological_process	response to prostaglandin E
A	0036064	cellular_component	ciliary basal body
A	0043434	biological_process	response to peptide hormone
A	0045542	biological_process	positive regulation of cholesterol biosynthetic process
A	0046676	biological_process	negative regulation of insulin secretion
A	0046872	molecular_function	metal ion binding
A	0051301	biological_process	cell division
A	0060236	biological_process	regulation of mitotic spindle organization
A	0070062	cellular_component	extracellular exosome
A	0070098	biological_process	chemokine-mediated signaling pathway
A	0072678	biological_process	T cell migration
A	1901082	biological_process	positive regulation of relaxation of smooth muscle
A	1904322	biological_process	cellular response to forskolin
A	1904778	biological_process	positive regulation of protein localization to cell cortex
B	0000166	molecular_function	nucleotide binding
B	0000287	molecular_function	magnesium ion binding
B	0001664	molecular_function	G protein-coupled receptor binding
B	0003924	molecular_function	GTPase activity
B	0003925	molecular_function	G protein activity
B	0005515	molecular_function	protein binding
B	0005525	molecular_function	GTP binding
B	0005634	cellular_component	nucleus
B	0005654	cellular_component	nucleoplasm
B	0005730	cellular_component	nucleolus
B	0005737	cellular_component	cytoplasm
B	0005765	cellular_component	lysosomal membrane
B	0005794	cellular_component	Golgi apparatus
B	0005813	cellular_component	centrosome
B	0005829	cellular_component	cytosol
B	0005834	cellular_component	heterotrimeric G-protein complex
B	0005856	cellular_component	cytoskeleton
B	0005886	cellular_component	plasma membrane
B	0005938	cellular_component	cell cortex
B	0007165	biological_process	signal transduction
B	0007186	biological_process	G protein-coupled receptor signaling pathway
B	0007188	biological_process	adenylate cyclase-modulating G protein-coupled receptor signaling pathway
B	0007189	biological_process	adenylate cyclase-activating G protein-coupled receptor signaling pathway
B	0007193	biological_process	adenylate cyclase-inhibiting G protein-coupled receptor signaling pathway
B	0007198	biological_process	adenylate cyclase-inhibiting serotonin receptor signaling pathway
B	0007218	biological_process	neuropeptide signaling pathway
B	0010854	molecular_function	adenylate cyclase regulator activity
B	0010855	molecular_function	adenylate cyclase inhibitor activity
B	0016020	cellular_component	membrane
B	0016787	molecular_function	hydrolase activity
B	0019001	molecular_function	guanyl nucleotide binding
B	0019003	molecular_function	GDP binding
B	0030496	cellular_component	midbody
B	0031683	molecular_function	G-protein beta/gamma-subunit complex binding
B	0031749	molecular_function	D2 dopamine receptor binding
B	0031821	molecular_function	G protein-coupled serotonin receptor binding
B	0034451	cellular_component	centriolar satellite
B	0034695	biological_process	response to prostaglandin E
B	0036064	cellular_component	ciliary basal body
B	0043434	biological_process	response to peptide hormone
B	0045542	biological_process	positive regulation of cholesterol biosynthetic process
B	0046676	biological_process	negative regulation of insulin secretion
B	0046872	molecular_function	metal ion binding
B	0051301	biological_process	cell division
B	0060236	biological_process	regulation of mitotic spindle organization
B	0070062	cellular_component	extracellular exosome
B	0070098	biological_process	chemokine-mediated signaling pathway
B	0072678	biological_process	T cell migration
B	1901082	biological_process	positive regulation of relaxation of smooth muscle
B	1904322	biological_process	cellular response to forskolin
B	1904778	biological_process	positive regulation of protein localization to cell cortex
C	0030695	molecular_function	GTPase regulator activity
D	0030695	molecular_function	GTPase regulator activity

Functional Information from PDB Data

site_id	AC1
Number of Residues	19
Details	BINDING SITE FOR RESIDUE GDP A 1348

Chain	Residue
A	GLY42
A	ARG176
A	ARG178
A	ASN269
A	LYS270
A	ASP272
A	LEU273
A	CYS325
A	ALA326
A	THR327
C	ARG516
A	GLU43
A	SER44
A	GLY45
A	LYS46
A	SER47
A	THR48
A	ASP150
A	SER151

site_id	AC2
Number of Residues	19
Details	BINDING SITE FOR RESIDUE GDP B 1348

Chain	Residue
B	GLY42
B	GLU43
B	SER44
B	GLY45
B	LYS46
B	SER47
B	THR48
B	ASP150
B	SER151
B	ARG176
B	ARG178
B	ASN269
B	LYS270
B	ASP272
B	LEU273
B	CYS325
B	ALA326
B	THR327
D	ARG516

site_id	AC3
Number of Residues	5
Details	BINDING SITE FOR RESIDUE SO4 C 1535

Chain	Residue
A	GLN147
A	ARG242
C	SER510
C	GLY511
C	HIS513

site_id	AC4
Number of Residues	5
Details	BINDING SITE FOR RESIDUE SO4 D 1535

Chain	Residue
B	GLN147
B	ARG242
D	SER510
D	GLY511
D	HIS513

site_id	AC5
Number of Residues	9
Details	BINDING SITE FOR RESIDUE SRT B 1349

Chain	Residue
A	GLU275
A	THR295
A	GLU297
B	LYS271
B	HIS322
B	PHE323
B	ASN331
B	PHE334
B	VAL335

site_id	AC6
Number of Residues	9
Details	BINDING SITE FOR RESIDUE SRT A 1349

Chain	Residue
A	LYS271
A	HIS322
A	PHE323
A	ASN331
A	PHE334
B	GLU275
B	THR295
B	TYR296
B	GLU297

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	26
Details	Region: {"description":"G1 motif","evidences":[{"source":"PROSITE-ProRule","id":"PRU01230","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

site_id	SWS_FT_FI2
Number of Residues	16
Details	Region: {"description":"G2 motif","evidences":[{"source":"PROSITE-ProRule","id":"PRU01230","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

site_id	SWS_FT_FI3
Number of Residues	18
Details	Region: {"description":"G3 motif","evidences":[{"source":"PROSITE-ProRule","id":"PRU01230","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

site_id	SWS_FT_FI4
Number of Residues	14
Details	Region: {"description":"G4 motif","evidences":[{"source":"PROSITE-ProRule","id":"PRU01230","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

site_id	SWS_FT_FI5
Number of Residues	10
Details	Region: {"description":"G5 motif","evidences":[{"source":"PROSITE-ProRule","id":"PRU01230","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

site_id	SWS_FT_FI6
Number of Residues	16
Details	Binding site: {"evidences":[{"source":"PubMed","id":"21115486","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"1KJY","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1Y3A","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"2G83","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"2GTP","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"2IK8","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"2OM2","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"2XNS","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3ONW","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3QE0","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3QI2","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3UMR","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3UMS","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"4G5Q","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI7
Number of Residues	4
Details	Binding site: {"evidences":[{"source":"PubMed","id":"18434541","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"22383884","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"3QE0","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI8
Number of Residues	2
Details	Binding site: {"evidences":[{"source":"PDB","id":"1KJY","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"2OM2","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"2XNS","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3ONW","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3QE0","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3QI2","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"4G5Q","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI9
Number of Residues	12
Details	Binding site: {"evidences":[{"source":"PDB","id":"1KJY","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1Y3A","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"2G83","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"2GTP","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"2IK8","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"2OM2","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"2XNS","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3ONW","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3QE0","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3QI2","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3UMR","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3UMS","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"4G5Q","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI10
Number of Residues	8
Details	Binding site: {"evidences":[{"source":"PubMed","id":"21115486","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

site_id	SWS_FT_FI11
Number of Residues	2
Details	Binding site: {"evidences":[{"source":"PDB","id":"1Y3A","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"2G83","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"2GTP","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"2IK8","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"2OM2","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3ONW","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3QE0","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3QI2","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3UMR","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3UMS","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"4G5Q","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI12
Number of Residues	2
Details	Modified residue: {"description":"ADP-ribosylarginine; by cholera toxin","evidences":[{"evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

site_id	SWS_FT_FI13
Number of Residues	2
Details	Modified residue: {"description":"Deamidated glutamine; by Photorhabdus PAU_02230","evidences":[{"source":"PubMed","id":"24141704","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

site_id	SWS_FT_FI14
Number of Residues	46
Details	Domain: {"description":"GoLoco","evidences":[{"source":"PROSITE-ProRule","id":"PRU00097","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)
		EDMap 2fo-fc (MTZ)