2XHI
Separation-of-function mutants unravel the dual reaction mode of human 8-oxoguanine DNA glycosylase
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A | N-GLYCOSYLASE/DNA LYASE | polymer | 360 | 40566.0 | 1 | UniProt (O15527) Pfam (PF07934) Pfam (PF00730) In PDB | HOMO SAPIENS (HUMAN) | 8-OXOGUANINE DNA GLYCOSYLASE, DNA-(APURINIC OR APYRIMIDINIC SITE) LYASE, AP LYASE |
2 | B | 5'-D(*GP*GP*TP*AP*GP*AP*CP*CP*TP*GP*GP*AP*CP*GP*C)-3' | polymer | 15 | 4635.0 | 1 | |||
3 | C | 5'-D(*GP*CP*GP*TP*CP*CP*AP*(8OG)P*GP*TP*CP*TP*AP*CP*C)-3' | polymer | 15 | 4561.9 | 1 | |||
4 | A, C | CALCIUM ION | non-polymer | 40.1 | 2 | Chemie (CA) | |||
5 | water | water | 18.0 | 399 | Chemie (HOH) |
Sequence modifications
A: 1 - 345 (UniProt: O15527)
PDB | External Database | Details |
---|---|---|
Met -14 | - | expression tag |
Gly -13 | - | expression tag |
Ser -12 | - | expression tag |
His -11 | - | expression tag |
His -10 | - | expression tag |
His -9 | - | expression tag |
His -8 | - | expression tag |
His -7 | - | expression tag |
His -6 | - | expression tag |
Ser -5 | - | expression tag |
Gln -4 | - | expression tag |
Asp -3 | - | expression tag |
Pro -2 | - | expression tag |
Asn -1 | - | expression tag |
Ser 0 | - | expression tag |
Cys 249 | Lys 249 | engineered mutation |
Lys 253 | Cys 253 | engineered mutation |
Asn 268 | Asp 268 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 3 |
Total formula weight | 49762.9 | |
Non-Polymers* | Number of molecules | 2 |
Total formula weight | 80.2 | |
All* | Total formula weight | 49843.1 |