2XHI

Separation-of-function mutants unravel the dual reaction mode of human 8-oxoguanine DNA glycosylase

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	N-GLYCOSYLASE/DNA LYASE	polymer	360	40566.0	1	UniProt (O15527) Pfam (PF07934) Pfam (PF00730)	HOMO SAPIENS (HUMAN)	8-OXOGUANINE DNA GLYCOSYLASE, DNA-(APURINIC OR APYRIMIDINIC SITE) LYASE, AP LYASE
2	B (B)	5'-D(*GP*GP*TP*AP*GP*AP*CP*CP*TP*GP*GP*AP*CP*GP*C)-3'	polymer	15	4635.0	1
3	C (C)	5'-D(*GP*CP*GP*TP*CP*CP*AP*(8OG)P*GP*TP*CP*TP*AP*CP*C)-3'	polymer	15	4561.9	1
4	D, E (A, C)	CALCIUM ION	non-polymer		40.1	2	Chemie (CA)
5	F, G, H (A, B, C)	water	water		18.0	399	Chemie (HOH)

Sequence modifications

A: 1 - 345 (UniProt: O15527)

PDB	External Database	Details
Met -14	-	expression tag
Gly -13	-	expression tag
Ser -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
Ser -5	-	expression tag
Gln -4	-	expression tag
Asp -3	-	expression tag
Pro -2	-	expression tag
Asn -1	-	expression tag
Ser 0	-	expression tag
Cys 249	Lys 249	engineered mutation
Lys 253	Cys 253	engineered mutation
Asn 268	Asp 268	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	49762.9
Non-Polymers*	Number of molecules	2
	Total formula weight	80.2
All*	Total formula weight	49843.1

*Water molecules are not included.