2XHI
Separation-of-function mutants unravel the dual reaction mode of human 8-oxoguanine DNA glycosylase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 92.669, 92.669, 210.986 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 24.630 - 1.550 |
| R-factor | 0.23113 |
| Rwork | 0.229 |
| R-free | 0.26472 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1yqr |
| RMSD bond length | 0.025 |
| RMSD bond angle | 2.291 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.400 | 1.630 |
| High resolution limit [Å] | 1.550 | 1.550 |
| Number of reflections | 77880 | |
| <I/σ(I)> | 14.5 | 2.3 |
| Completeness [%] | 99.5 | 99.6 |
| Redundancy | 5.8 | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
