2XGE

Crystal structure of a designed heterodimeric variant T-A(A)B of the tetracycline repressor

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	TETRACYCLINE REPRESSOR PROTEIN CLASS B FROM TRANSPOSON TN10, TETRACYCLINE REPRESSOR PROTEIN CLASS D	polymer	208	23444.6	1	UniProt (P04483) UniProt (P0ACT4) Pfam (PF00440) Pfam (PF02909)	ESCHERICHIA COLI
2	B (B)	TETRACYCLINE REPRESSOR PROTEIN CLASS B FROM TRANSPOSON TN10, TETRACYCLINE REPRESSOR PROTEIN CLASS D	polymer	211	23710.9	1	UniProt (P04483) UniProt (P0ACT4) Pfam (PF00440) Pfam (PF02909)	ESCHERICHIA COLI
3	C (A)	1,2-ETHANEDIOL	non-polymer		62.1	1	Chemie (EDO)
4	D (A)	SODIUM ION	non-polymer		23.0	1	Chemie (NA)
5	E (A)	CHLORIDE ION	non-polymer		35.5	1	Chemie (CL)
6	F (A)	water	water		18.0	106	Chemie (HOH)

Sequence modifications

A: 1 - 50 (UniProt: P04483)
A: 51 - 208 (UniProt: P0ACT4)

PDB	External Database	Details
Trp 136	Ala 136	engineered mutation
Ala 193	Leu 193	engineered mutation
Ile 197	Phe 197	engineered mutation

B: 1 - 50 (UniProt: P04483)

PDB	External Database	Details
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag

B: 51 - 208 (UniProt: P0ACT4)

PDB	External Database	Details
Ala 140	Phe 140	engineered mutation
Trp 192	Ser 192	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	47155.6
Non-Polymers*	Number of molecules	3
	Total formula weight	120.5
All*	Total formula weight	47276.1

*Water molecules are not included.