2XGE

Crystal structure of a designed heterodimeric variant T-A(A)B of the tetracycline repressor

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Detector	MARRESEARCH
Spacegroup name	I 41 2 2
Unit cell lengths	69.127, 69.127, 182.949
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.960 - 2.140
R-factor	0.2097
Rwork	0.205
R-free	0.26000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1a6i
RMSD bond length	0.012
RMSD bond angle	1.378
Data reduction software	XDS
Data scaling software	XSCALE
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	2.230	2.230
High resolution limit [Å]	2.100	2.100
Rmerge	0.060	0.540
Number of reflections	13364
<I/σ(I)>	28.6	5.6
Completeness [%]	99.6	99.3
Redundancy	13.6	13.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1