2WQ8

Glycan labelling using engineered variants of galactose oxidase obtained by directed evolution

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	GALACTOSE OXIDASE	polymer	661	71188.5	1	UniProt (Q01745) UniProt (by SIFTS) (P0CS93)	GIBBERELLA ZEAE	GAO, GOASE, GO
2	B (A)	COPPER (II) ION	non-polymer		63.5	1	Chemie (CU)
3	C (A)	CALCIUM ION	non-polymer		40.1	1	Chemie (CA)
4	D, E (A)	ACETIC ACID	non-polymer		60.1	2	Chemie (ACY)
5	F, G, H, I, J... (A)	1,2-ETHANEDIOL	non-polymer		62.1	9	Chemie (EDO)
6	O, P, Q (A)	GLYCEROL	non-polymer		92.1	3	Chemie (GOL)
7	R (A)	water	water		18.0	382	Chemie (HOH)

Sequence modifications

A: 1 - 639 (UniProt: Q01745)

PDB	External Database	Details
Met -21	-	expression tag
Gly -20	-	expression tag
His -19	-	expression tag
His -18	-	expression tag
His -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
His -6	-	expression tag
Ile -5	-	expression tag
Glu -4	-	expression tag
Gly -3	-	expression tag
Arg -2	-	expression tag
His -1	-	expression tag
Met 0	-	expression tag
Pro 10	Ser 51	engineered mutation
Val 70	Met 111	engineered mutation
Glu 195	Gly 236	engineered mutation
Phe 290	Trp 331	engineered mutation
Lys 330	Arg 371	engineered mutation
Thr 406	Gln 447	engineered mutation
Ile 463	Pro 504	engineered mutation
Ala 494	Val 535	engineered mutation
Asp 535	Asn 576	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	71188.5
Non-Polymers*	Number of molecules	16
	Total formula weight	1058.6
All*	Total formula weight	72247.1

*Water molecules are not included.