2WQ8
Glycan labelling using engineered variants of galactose oxidase obtained by directed evolution
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A | GALACTOSE OXIDASE | polymer | 661 | 71188.5 | 1 | UniProt (Q01745) Pfam (PF00754) Pfam (PF01344) Pfam (PF09118) UniProt (by SIFTS) (P0CS93) In PDB | GIBBERELLA ZEAE | GAO, GOASE, GO |
2 | A | COPPER (II) ION | non-polymer | 63.5 | 1 | Chemie (CU) | |||
3 | A | CALCIUM ION | non-polymer | 40.1 | 1 | Chemie (CA) | |||
4 | A | ACETIC ACID | non-polymer | 60.1 | 2 | Chemie (ACY) | |||
5 | A | 1,2-ETHANEDIOL | non-polymer | 62.1 | 9 | Chemie (EDO) | |||
6 | A | GLYCEROL | non-polymer | 92.1 | 3 | Chemie (GOL) | |||
7 | water | water | 18.0 | 382 | Chemie (HOH) |
Sequence modifications
A: 1 - 639 (UniProt: Q01745)
PDB | External Database | Details |
---|---|---|
Met -21 | - | expression tag |
Gly -20 | - | expression tag |
His -19 | - | expression tag |
His -18 | - | expression tag |
His -17 | - | expression tag |
His -16 | - | expression tag |
His -15 | - | expression tag |
His -14 | - | expression tag |
His -13 | - | expression tag |
His -12 | - | expression tag |
His -11 | - | expression tag |
His -10 | - | expression tag |
Ser -9 | - | expression tag |
Ser -8 | - | expression tag |
Gly -7 | - | expression tag |
His -6 | - | expression tag |
Ile -5 | - | expression tag |
Glu -4 | - | expression tag |
Gly -3 | - | expression tag |
Arg -2 | - | expression tag |
His -1 | - | expression tag |
Met 0 | - | expression tag |
Pro 10 | Ser 51 | engineered mutation |
Val 70 | Met 111 | engineered mutation |
Glu 195 | Gly 236 | engineered mutation |
Phe 290 | Trp 331 | engineered mutation |
Lys 330 | Arg 371 | engineered mutation |
Thr 406 | Gln 447 | engineered mutation |
Ile 463 | Pro 504 | engineered mutation |
Ala 494 | Val 535 | engineered mutation |
Asp 535 | Asn 576 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 1 |
Total formula weight | 71188.5 | |
Non-Polymers* | Number of molecules | 16 |
Total formula weight | 1058.6 | |
All* | Total formula weight | 72247.1 |