2WQ8

Glycan labelling using engineered variants of galactose oxidase obtained by directed evolution

Summary for 2WQ8

Entry DOI	10.2210/pdb2wq8/pdb
Related	1GOF 1GOG 1GOH 1K3I 1T2X 2JKX 2VZ1 2VZ3
Descriptor	GALACTOSE OXIDASE, COPPER (II) ION, CALCIUM ION, ... (7 entities in total)
Functional Keywords	oxidoreductase, kelch repeat, metal-binding, glycoengineering, directed evolution
Biological source	GIBBERELLA ZEAE
Total number of polymer chains	1
Total formula weight	72247.10
Authors	Rannes, J.G.,Ioannou, A.,Willies, S.C.,Behrens, C.,Grogan, G.J.,Flitsch, S.L.,Turner, N.J. (deposition date: 2009-08-14, release date: 2010-09-01, Last modification date: 2024-10-09)
Primary citation	Rannes, J.B.,Ioannou, A.,Willies, S.C.,Grogan, G.,Behrens, C.,Flitsch, S.L.,Turner, N.J. Glycoprotein Labeling Using Engineered Variants of Galactose Oxidase Obtained by Directed Evolution. J.Am.Chem.Soc., 133:8436-, 2011 Cited by PubMed Abstract: A directed evolution approach has been used for the generation of variants of galactose oxidase (GOase) that can selectively oxidize glycans on glycoproteins. The aldehyde function introduced on the glycans D-mannose (Man) and D-N-acetyl glucosamine (GlcNAc) by the enzyme variants could then be used to label the glycoproteins and also whole cells that display mannosides on their surface. PubMed: 21526835 DOI: 10.1021/JA2018477 PDB entries with the same primary citation
Experimental method	X-RAY DIFFRACTION (2.19 Å)

Structure validation