2W83

Crystal structure of the ARF6 GTPase in complex with a specific effector, JIP4

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, E	ADP-RIBOSYLATION FACTOR 6	polymer	165	18990.8	3	UniProt (P62330) Pfam (PF00025) In PDB	HOMO SAPIENS (HUMAN)	ARF6
2	C, D	C-JUN-AMINO-TERMINAL KINASE-INTERACTING PROTEIN 4	polymer	77	8878.2	2	UniProt (O60271) Pfam (PF16471) In PDB	HOMO SAPIENS (HUMAN)	JIP4, JNK-INTERACTING PROTEIN 4, JIP-4, JLP, HLC-6, PHET, CT89, JNK-ASSOCIATED LEUCINE-ZIPPER PROTEIN, SPERM-ASSOCIATED ANTIGEN 9, MITOGEN-ACTIVATED PROTEIN KINASE 8-INTERACTING PROTEIN 4, HUMAN LUNG CANCER ONCOGENE 6 PROTEIN, PROLIFERATION-INDUCING PROTEIN 6, SPERM-SPECIFIC PROTEIN, SPERM SURFACE PROTEIN, PROTEIN HIGHLY EXPRESSED IN TESTIS, SUNDAY DRIVER 1, CANCER/TESTIS ANTIGEN 89
3	A, B, E	GUANOSINE-5'-TRIPHOSPHATE	non-polymer		523.2	3	Chemie (GTP)
4	A, B, E	MAGNESIUM ION	non-polymer		24.3	3	Chemie (MG)
5	A, C, E	1,4-DIETHYLENE DIOXIDE	non-polymer		88.1	6	Chemie (DIO)
6	A	GLYCEROL	non-polymer		92.1	1	Chemie (GOL)
7		water	water		18.0	274	Chemie (HOH)

Sequence modifications

A, B, E: 11 - 12 (PDB: 2W83)
A, B, E: 13 - 175 (UniProt: P62330)

PDB	External Database	Details
Leu 67	Gln 67	engineered mutation

C, D: 386 - 391 (PDB: 2W83)
C, D: 392 - 462 (UniProt: O60271)

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	74728.8
Non-Polymers*	Number of molecules	13
	Total formula weight	2263.2
All*	Total formula weight	76992.0

*Water molecules are not included.