Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

2W83

Crystal structure of the ARF6 GTPase in complex with a specific effector, JIP4

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0001726	cellular_component	ruffle
A	0001889	biological_process	liver development
A	0003924	molecular_function	GTPase activity
A	0003925	molecular_function	G protein activity
A	0005515	molecular_function	protein binding
A	0005525	molecular_function	GTP binding
A	0005737	cellular_component	cytoplasm
A	0005768	cellular_component	endosome
A	0005769	cellular_component	early endosome
A	0005794	cellular_component	Golgi apparatus
A	0005829	cellular_component	cytosol
A	0005886	cellular_component	plasma membrane
A	0005925	cellular_component	focal adhesion
A	0005938	cellular_component	cell cortex
A	0006886	biological_process	intracellular protein transport
A	0007155	biological_process	cell adhesion
A	0007399	biological_process	nervous system development
A	0010975	biological_process	regulation of neuron projection development
A	0010976	biological_process	positive regulation of neuron projection development
A	0015031	biological_process	protein transport
A	0016020	cellular_component	membrane
A	0016192	biological_process	vesicle-mediated transport
A	0016787	molecular_function	hydrolase activity
A	0019003	molecular_function	GDP binding
A	0030139	cellular_component	endocytic vesicle
A	0030154	biological_process	cell differentiation
A	0030496	cellular_component	midbody
A	0030838	biological_process	positive regulation of actin filament polymerization
A	0030866	biological_process	cortical actin cytoskeleton organization
A	0031527	cellular_component	filopodium membrane
A	0031901	cellular_component	early endosome membrane
A	0031996	molecular_function	thioesterase binding
A	0032154	cellular_component	cleavage furrow
A	0032456	biological_process	endocytic recycling
A	0034394	biological_process	protein localization to cell surface
A	0035020	biological_process	regulation of Rac protein signal transduction
A	0035591	molecular_function	signaling adaptor activity
A	0036010	biological_process	protein localization to endosome
A	0042995	cellular_component	cell projection
A	0048261	biological_process	negative regulation of receptor-mediated endocytosis
A	0048488	biological_process	synaptic vesicle endocytosis
A	0050714	biological_process	positive regulation of protein secretion
A	0051301	biological_process	cell division
A	0051489	biological_process	regulation of filopodium assembly
A	0051549	biological_process	positive regulation of keratinocyte migration
A	0055037	cellular_component	recycling endosome
A	0055038	cellular_component	recycling endosome membrane
A	0060998	biological_process	regulation of dendritic spine development
A	0070062	cellular_component	extracellular exosome
A	0072659	biological_process	protein localization to plasma membrane
A	0090162	biological_process	establishment of epithelial cell polarity
A	0090543	cellular_component	Flemming body
A	0097178	biological_process	ruffle assembly
A	0097284	biological_process	hepatocyte apoptotic process
A	0098793	cellular_component	presynapse
A	0098794	cellular_component	postsynapse
A	0098978	cellular_component	glutamatergic synapse
A	0099562	biological_process	maintenance of postsynaptic density structure
A	0120183	biological_process	positive regulation of focal adhesion disassembly
A	1902217	biological_process	erythrocyte apoptotic process
A	1903078	biological_process	positive regulation of protein localization to plasma membrane
A	1903438	biological_process	positive regulation of mitotic cytokinetic process
A	1905345	biological_process	protein localization to cleavage furrow
A	1905606	biological_process	regulation of presynapse assembly
A	1990090	biological_process	cellular response to nerve growth factor stimulus
A	2000009	biological_process	negative regulation of protein localization to cell surface
A	2000171	biological_process	negative regulation of dendrite development
B	0001726	cellular_component	ruffle
B	0001889	biological_process	liver development
B	0003924	molecular_function	GTPase activity
B	0003925	molecular_function	G protein activity
B	0005515	molecular_function	protein binding
B	0005525	molecular_function	GTP binding
B	0005737	cellular_component	cytoplasm
B	0005768	cellular_component	endosome
B	0005769	cellular_component	early endosome
B	0005794	cellular_component	Golgi apparatus
B	0005829	cellular_component	cytosol
B	0005886	cellular_component	plasma membrane
B	0005925	cellular_component	focal adhesion
B	0005938	cellular_component	cell cortex
B	0006886	biological_process	intracellular protein transport
B	0007155	biological_process	cell adhesion
B	0007399	biological_process	nervous system development
B	0010975	biological_process	regulation of neuron projection development
B	0010976	biological_process	positive regulation of neuron projection development
B	0015031	biological_process	protein transport
B	0016020	cellular_component	membrane
B	0016192	biological_process	vesicle-mediated transport
B	0016787	molecular_function	hydrolase activity
B	0019003	molecular_function	GDP binding
B	0030139	cellular_component	endocytic vesicle
B	0030154	biological_process	cell differentiation
B	0030496	cellular_component	midbody
B	0030838	biological_process	positive regulation of actin filament polymerization
B	0030866	biological_process	cortical actin cytoskeleton organization
B	0031527	cellular_component	filopodium membrane
B	0031901	cellular_component	early endosome membrane
B	0031996	molecular_function	thioesterase binding
B	0032154	cellular_component	cleavage furrow
B	0032456	biological_process	endocytic recycling
B	0034394	biological_process	protein localization to cell surface
B	0035020	biological_process	regulation of Rac protein signal transduction
B	0035591	molecular_function	signaling adaptor activity
B	0036010	biological_process	protein localization to endosome
B	0042995	cellular_component	cell projection
B	0048261	biological_process	negative regulation of receptor-mediated endocytosis
B	0048488	biological_process	synaptic vesicle endocytosis
B	0050714	biological_process	positive regulation of protein secretion
B	0051301	biological_process	cell division
B	0051489	biological_process	regulation of filopodium assembly
B	0051549	biological_process	positive regulation of keratinocyte migration
B	0055037	cellular_component	recycling endosome
B	0055038	cellular_component	recycling endosome membrane
B	0060998	biological_process	regulation of dendritic spine development
B	0070062	cellular_component	extracellular exosome
B	0072659	biological_process	protein localization to plasma membrane
B	0090162	biological_process	establishment of epithelial cell polarity
B	0090543	cellular_component	Flemming body
B	0097178	biological_process	ruffle assembly
B	0097284	biological_process	hepatocyte apoptotic process
B	0098793	cellular_component	presynapse
B	0098794	cellular_component	postsynapse
B	0098978	cellular_component	glutamatergic synapse
B	0099562	biological_process	maintenance of postsynaptic density structure
B	0120183	biological_process	positive regulation of focal adhesion disassembly
B	1902217	biological_process	erythrocyte apoptotic process
B	1903078	biological_process	positive regulation of protein localization to plasma membrane
B	1903438	biological_process	positive regulation of mitotic cytokinetic process
B	1905345	biological_process	protein localization to cleavage furrow
B	1905606	biological_process	regulation of presynapse assembly
B	1990090	biological_process	cellular response to nerve growth factor stimulus
B	2000009	biological_process	negative regulation of protein localization to cell surface
B	2000171	biological_process	negative regulation of dendrite development
C	0005078	molecular_function	MAP-kinase scaffold activity
D	0005078	molecular_function	MAP-kinase scaffold activity
E	0001726	cellular_component	ruffle
E	0001889	biological_process	liver development
E	0003924	molecular_function	GTPase activity
E	0003925	molecular_function	G protein activity
E	0005515	molecular_function	protein binding
E	0005525	molecular_function	GTP binding
E	0005737	cellular_component	cytoplasm
E	0005768	cellular_component	endosome
E	0005769	cellular_component	early endosome
E	0005794	cellular_component	Golgi apparatus
E	0005829	cellular_component	cytosol
E	0005886	cellular_component	plasma membrane
E	0005925	cellular_component	focal adhesion
E	0005938	cellular_component	cell cortex
E	0006886	biological_process	intracellular protein transport
E	0007155	biological_process	cell adhesion
E	0007399	biological_process	nervous system development
E	0010975	biological_process	regulation of neuron projection development
E	0010976	biological_process	positive regulation of neuron projection development
E	0015031	biological_process	protein transport
E	0016020	cellular_component	membrane
E	0016192	biological_process	vesicle-mediated transport
E	0016787	molecular_function	hydrolase activity
E	0019003	molecular_function	GDP binding
E	0030139	cellular_component	endocytic vesicle
E	0030154	biological_process	cell differentiation
E	0030496	cellular_component	midbody
E	0030838	biological_process	positive regulation of actin filament polymerization
E	0030866	biological_process	cortical actin cytoskeleton organization
E	0031527	cellular_component	filopodium membrane
E	0031901	cellular_component	early endosome membrane
E	0031996	molecular_function	thioesterase binding
E	0032154	cellular_component	cleavage furrow
E	0032456	biological_process	endocytic recycling
E	0034394	biological_process	protein localization to cell surface
E	0035020	biological_process	regulation of Rac protein signal transduction
E	0035591	molecular_function	signaling adaptor activity
E	0036010	biological_process	protein localization to endosome
E	0042995	cellular_component	cell projection
E	0048261	biological_process	negative regulation of receptor-mediated endocytosis
E	0048488	biological_process	synaptic vesicle endocytosis
E	0050714	biological_process	positive regulation of protein secretion
E	0051301	biological_process	cell division
E	0051489	biological_process	regulation of filopodium assembly
E	0051549	biological_process	positive regulation of keratinocyte migration
E	0055037	cellular_component	recycling endosome
E	0055038	cellular_component	recycling endosome membrane
E	0060998	biological_process	regulation of dendritic spine development
E	0070062	cellular_component	extracellular exosome
E	0072659	biological_process	protein localization to plasma membrane
E	0090162	biological_process	establishment of epithelial cell polarity
E	0090543	cellular_component	Flemming body
E	0097178	biological_process	ruffle assembly
E	0097284	biological_process	hepatocyte apoptotic process
E	0098793	cellular_component	presynapse
E	0098794	cellular_component	postsynapse
E	0098978	cellular_component	glutamatergic synapse
E	0099562	biological_process	maintenance of postsynaptic density structure
E	0120183	biological_process	positive regulation of focal adhesion disassembly
E	1902217	biological_process	erythrocyte apoptotic process
E	1903078	biological_process	positive regulation of protein localization to plasma membrane
E	1903438	biological_process	positive regulation of mitotic cytokinetic process
E	1905345	biological_process	protein localization to cleavage furrow
E	1905606	biological_process	regulation of presynapse assembly
E	1990090	biological_process	cellular response to nerve growth factor stimulus
E	2000009	biological_process	negative regulation of protein localization to cell surface
E	2000171	biological_process	negative regulation of dendrite development

Functional Information from PDB Data

site_id	AC1
Number of Residues	27
Details	BINDING SITE FOR RESIDUE GTP A 1173

Chain	Residue
A	ASP22
A	THR44
A	GLY65
A	GLY66
A	ASN122
A	LYS123
A	ASP125
A	LEU126
A	CYS155
A	ALA156
A	MG1174
A	ALA23
A	HOH2011
A	HOH2033
A	HOH2093
A	HOH2094
A	HOH2095
A	HOH2096
A	HOH2097
A	HOH2098
A	ALA24
A	GLY25
A	LYS26
A	THR27
A	THR28
A	THR41
A	PRO43

site_id	AC2
Number of Residues	5
Details	BINDING SITE FOR RESIDUE MG A 1174

Chain	Residue
A	THR27
A	THR44
A	GTP1173
A	HOH2033
A	HOH2094

site_id	AC3
Number of Residues	21
Details	BINDING SITE FOR RESIDUE GTP B 1173

Chain	Residue
B	ASP22
B	ALA23
B	ALA24
B	GLY25
B	LYS26
B	THR27
B	THR28
B	THR41
B	PRO43
B	THR44
B	GLY66
B	ASN122
B	LYS123
B	ASP125
B	CYS155
B	ALA156
B	THR157
B	MG1174
B	HOH2007
B	HOH2015
B	HOH2029

site_id	AC4
Number of Residues	5
Details	BINDING SITE FOR RESIDUE MG B 1174

Chain	Residue
B	THR27
B	THR44
B	GTP1173
B	HOH2007
B	HOH2015

site_id	AC5
Number of Residues	28
Details	BINDING SITE FOR RESIDUE GTP E 1173

Chain	Residue
E	ASP22
E	ALA23
E	ALA24
E	GLY25
E	LYS26
E	THR27
E	THR28
E	THR41
E	PRO43
E	THR44
E	GLY65
E	GLY66
E	ASN122
E	LYS123
E	ASP125
E	LEU126
E	CYS155
E	ALA156
E	THR157
E	MG1174
E	HOH2014
E	HOH2029
E	HOH2083
E	HOH2084
E	HOH2085
E	HOH2086
E	HOH2088
E	HOH2089

site_id	AC6
Number of Residues	5
Details	BINDING SITE FOR RESIDUE MG E 1174

Chain	Residue
E	THR27
E	THR44
E	GTP1173
E	HOH2029
E	HOH2083

site_id	AC7
Number of Residues	4
Details	BINDING SITE FOR RESIDUE DIO A 1175

Chain	Residue
A	PRO132
A	HIS133
A	GLN136
E	GLN136

site_id	AC8
Number of Residues	5
Details	BINDING SITE FOR RESIDUE DIO E 1175

Chain	Residue
E	HIS133
A	PRO132
A	HIS133
E	LYS131
E	PRO132

site_id	AC9
Number of Residues	6
Details	BINDING SITE FOR RESIDUE DIO C 1453

Chain	Residue
A	ILE70
C	THR404
C	ILE415
C	DIO1454
D	LEU399
D	GLU402

site_id	BC1
Number of Residues	5
Details	BINDING SITE FOR RESIDUE DIO C 1454

Chain	Residue
A	LYS69
A	LEU73
C	GLU408
C	ALA412
C	DIO1453

site_id	BC2
Number of Residues	6
Details	BINDING SITE FOR RESIDUE DIO E 1176

Chain	Residue
C	PHE391
E	THR27
E	LYS34
E	LEU35
E	THR41
E	ILE42

site_id	BC3
Number of Residues	5
Details	BINDING SITE FOR RESIDUE DIO E 1177

Chain	Residue
D	LEU407
D	ASN411
E	LYS69
E	ILE70
E	LEU73

site_id	BC4
Number of Residues	8
Details	BINDING SITE FOR RESIDUE GOL A 1176

Chain	Residue
A	ARG71
A	PRO72
A	TRP74
A	ARG75
A	TYR78
A	GLU102
A	ARG105
A	HOH2099

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	2
Details	MOD_RES: Phosphothreonine => ECO:0007744\|PubMed:17525332, ECO:0007744\|PubMed:18669648, ECO:0007744\|PubMed:20068231

Chain	Residue	Details
C	ASN418
D	ASN418
A	ASP63
A	ASN122
A	CYS155
B	ALA23
B	THR41
B	ASP63
B	ASN122
B	CYS155
E	ALA23
E	THR41
E	ASP63
E	ASN122
E	CYS155

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)
		EDMap 2fo-fc (MTZ)