2V7G

Crystal Structure of an Engineered Urocanase Tetramer

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	UROCANATE HYDRATASE	polymer	557	60811.7	4	UniProt (P25080) Pfam (PF17391) Pfam (PF01175) Pfam (PF17392)	PSEUDOMONAS PUTIDA	UROCANASE, IMIDAZOLONEPROPIONATE HYDROLASE
2	E, F, J, K, L... (A, B, C)	ACETATE ION	non-polymer		59.0	7	Chemie (ACT)
3	G, H, M, N, O... (A, B, D)	GLYCEROL	non-polymer		92.1	9	Chemie (GOL)
4	I, P, S, X (A, B, C, D)	NICOTINAMIDE-ADENINE-DINUCLEOTIDE	non-polymer		663.4	4	Chemie (NAD)
5	AA, BA, Y, Z (C, D, A, B)	water	water		18.0	1310	Chemie (HOH)

Sequence modifications

A, B, C, D: 1 - 1 (PDB: 2V7G)
A, B, C, D: 2 - 557 (UniProt: P25080)

PDB	External Database	Details
Ser 198	Cys 197	engineered mutation
Ile 277	Ala 276	engineered mutation
Ile 291	Glu 290	engineered mutation
Ala 301	Gln 300	engineered mutation
Ser 164	Thr 163	conflict
Leu 165	Val 164	conflict
Gly 167	Ala 166	conflict

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	243246.7
Non-Polymers*	Number of molecules	20
	Total formula weight	3895.9
All*	Total formula weight	247142.6

*Water molecules are not included.