Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

2V7G

Crystal Structure of an Engineered Urocanase Tetramer

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0005737	cellular_component	cytoplasm
A	0006547	biological_process	L-histidine metabolic process
A	0006548	biological_process	L-histidine catabolic process
A	0016153	molecular_function	urocanate hydratase activity
A	0016829	molecular_function	lyase activity
A	0019556	biological_process	L-histidine catabolic process to glutamate and formamide
A	0019557	biological_process	L-histidine catabolic process to glutamate and formate
B	0005737	cellular_component	cytoplasm
B	0006547	biological_process	L-histidine metabolic process
B	0006548	biological_process	L-histidine catabolic process
B	0016153	molecular_function	urocanate hydratase activity
B	0016829	molecular_function	lyase activity
B	0019556	biological_process	L-histidine catabolic process to glutamate and formamide
B	0019557	biological_process	L-histidine catabolic process to glutamate and formate
C	0005737	cellular_component	cytoplasm
C	0006547	biological_process	L-histidine metabolic process
C	0006548	biological_process	L-histidine catabolic process
C	0016153	molecular_function	urocanate hydratase activity
C	0016829	molecular_function	lyase activity
C	0019556	biological_process	L-histidine catabolic process to glutamate and formamide
C	0019557	biological_process	L-histidine catabolic process to glutamate and formate
D	0005737	cellular_component	cytoplasm
D	0006547	biological_process	L-histidine metabolic process
D	0006548	biological_process	L-histidine catabolic process
D	0016153	molecular_function	urocanate hydratase activity
D	0016829	molecular_function	lyase activity
D	0019556	biological_process	L-histidine catabolic process to glutamate and formamide
D	0019557	biological_process	L-histidine catabolic process to glutamate and formate

Functional Information from PDB Data

site_id	AC1
Number of Residues	36
Details	BINDING SITE FOR RESIDUE NAD A 3001

Chain	Residue
A	GLU44
A	GLY179
A	GLU197
A	SER198
A	GLN199
A	ARG202
A	GLY242
A	ASN243
A	ALA244
A	GLN264
A	THR265
A	TYR52
A	SER266
A	HIS268
A	GLY273
A	TYR274
A	LEU275
A	TRP281
A	TYR323
A	GLY324
A	ASN325
A	PHE345
A	GLY53
A	LEU445
A	ARG455
A	GLY493
A	HOH2344
A	HOH2345
A	HOH2346
A	HOH2347
A	GLY54
A	GLN131
A	ILE145
A	GLY176
A	GLY177
A	MET178

site_id	AC2
Number of Residues	36
Details	BINDING SITE FOR RESIDUE NAD B 3001

Chain	Residue
B	GLU44
B	TYR52
B	GLY53
B	GLY54
B	GLN131
B	ILE145
B	GLY176
B	GLY177
B	MET178
B	GLY179
B	GLU197
B	SER198
B	GLN199
B	ARG202
B	GLY242
B	ASN243
B	ALA244
B	GLN264
B	THR265
B	SER266
B	HIS268
B	GLY273
B	TYR274
B	LEU275
B	TRP281
B	TYR323
B	GLY324
B	ASN325
B	PHE345
B	LEU445
B	ARG455
B	GLY493
B	HOH2035
B	HOH2192
B	HOH2340
B	HOH2341

site_id	AC3
Number of Residues	29
Details	BINDING SITE FOR RESIDUE NAD C 3001

Chain	Residue
C	HOH2329
C	HOH2330
C	GLY176
C	GLY177
C	MET178
C	GLY179
C	GLU197
C	SER198
C	GLN199
C	ARG202
C	GLY242
C	ASN243
C	ALA244
C	GLN264
C	THR265
C	SER266
C	HIS268
C	GLY273
C	TYR274
C	LEU275
C	TRP281
C	TYR323
C	GLY324
C	ASN325
C	PHE345
C	ARG455
C	ACT1558
C	HOH2180
C	HOH2275

site_id	AC4
Number of Residues	30
Details	BINDING SITE FOR RESIDUE NAD D 3001

Chain	Residue
D	GLY176
D	GLY177
D	MET178
D	GLY179
D	GLU197
D	SER198
D	GLN199
D	ARG202
D	GLY242
D	ASN243
D	ALA244
D	GLN264
D	THR265
D	SER266
D	HIS268
D	GLY273
D	TYR274
D	LEU275
D	TYR323
D	GLY324
D	ASN325
D	PHE345
D	ARG455
D	HOH2241
D	HOH2287
D	HOH2288
D	HOH2289
D	HOH2290
D	HOH2291
D	HOH2292

site_id	AC5
Number of Residues	4
Details	BINDING SITE FOR RESIDUE ACT C 1558

Chain	Residue
C	HIS268
C	GLY324
C	ARG455
C	NAD3001

site_id	AC6
Number of Residues	6
Details	BINDING SITE FOR RESIDUE ACT B 1558

Chain	Residue
B	LYS87
B	HOH2332
B	HOH2333
D	ASP535
D	MET554
D	ILE555

site_id	AC7
Number of Residues	4
Details	BINDING SITE FOR RESIDUE ACT B 1559

Chain	Residue
B	VAL91
B	HOH2334
B	HOH2335
D	ASP551

site_id	AC8
Number of Residues	10
Details	BINDING SITE FOR RESIDUE ACT A 1558

Chain	Residue
A	TYR52
A	THR133
A	TYR139
A	GLY144
A	ILE145
A	MET178
A	ARG362
A	ASP443
A	HOH2127
A	HOH2291

site_id	AC9
Number of Residues	10
Details	BINDING SITE FOR RESIDUE ACT B 1560

Chain	Residue
B	TYR52
B	THR133
B	TYR139
B	GLY144
B	ILE145
B	MET178
B	ARG362
B	ASP443
B	HOH2275
B	HOH2336

site_id	BC1
Number of Residues	3
Details	BINDING SITE FOR RESIDUE ACT A 1559

Chain	Residue
A	GLN199
A	HOH2342
A	HOH2343

site_id	BC2
Number of Residues	7
Details	BINDING SITE FOR RESIDUE ACT C 1559

Chain	Residue
C	TYR52
C	THR133
C	TYR139
C	GLY144
C	ARG362
C	HOH2112
C	HOH2326

site_id	BC3
Number of Residues	4
Details	BINDING SITE FOR RESIDUE GOL B 1561

Chain	Residue
B	PRO453
B	ASN454
B	ASP465
B	HOH2220

site_id	BC4
Number of Residues	6
Details	BINDING SITE FOR RESIDUE GOL D 1558

Chain	Residue
D	SER198
D	GLY242
D	ASN243
D	LEU275
D	THR280
D	TRP281

site_id	BC5
Number of Residues	5
Details	BINDING SITE FOR RESIDUE GOL B 1562

Chain	Residue
B	GLU151
B	ARG158
B	HIS389
B	HOH2337
B	HOH2338

site_id	BC6
Number of Residues	3
Details	BINDING SITE FOR RESIDUE GOL D 1559

Chain	Residue
A	HOH2202
D	GLU246
D	GLY278

site_id	BC7
Number of Residues	8
Details	BINDING SITE FOR RESIDUE GOL A 1560

Chain	Residue
A	ILE277
A	TRP279
A	ALA298
A	SER302
D	PRO276
D	ILE277
D	TRP279
D	SER302

site_id	BC8
Number of Residues	4
Details	BINDING SITE FOR RESIDUE GOL D 1560

Chain	Residue
D	SER198
D	GLN200
D	SER201
D	HIS241

site_id	BC9
Number of Residues	6
Details	BINDING SITE FOR RESIDUE GOL B 1563

Chain	Residue
B	HIS160
B	GLN314
B	THR320
B	PRO343
B	TYR349
B	HOH2339

site_id	CC1
Number of Residues	4
Details	BINDING SITE FOR RESIDUE GOL A 1561

Chain	Residue
A	ARG356
A	ASP387
A	TRP412
A	LEU417

site_id	CC2
Number of Residues	4
Details	BINDING SITE FOR RESIDUE GOL D 1561

Chain	Residue
D	GLU151
D	ARG158
D	LEU390
D	TRP412

Functional Information from PROSITE/UniProt

site_id	PS01233
Number of Residues	16
Details	UROCANASE Urocanase signature. RDHlDsGSvsSPnRET

Chain	Residue	Details
A	ARG442-THR457

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	4
Details	Active site: {"evidences":[{"source":"HAMAP-Rule","id":"MF_00577","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"7901006","evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

site_id	SWS_FT_FI2
Number of Residues	44
Details	Binding site: {"evidences":[{"source":"HAMAP-Rule","id":"MF_00577","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"15313616","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"18187656","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"1UWK","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1UWL","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1W1U","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"2V7G","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI3
Number of Residues	20
Details	Binding site: {"evidences":[{"source":"PubMed","id":"15313616","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"18187656","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"1UWK","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1UWL","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1W1U","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"2V7G","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI4
Number of Residues	4
Details	Binding site: {"evidences":[{"source":"PubMed","id":"15313616","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"18187656","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"1UWK","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1UWL","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"2V7G","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI5
Number of Residues	4
Details	Binding site: {"evidences":[{"source":"PubMed","id":"15313616","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"18187656","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"1W1U","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"2V7G","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)
		EDMap 2fo-fc (MTZ)