2V7G
Crystal Structure of an Engineered Urocanase Tetramer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-11-01 |
| Detector | MARREASEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 68.068, 154.316, 216.391 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.810 - 2.000 |
| R-factor | 0.156 |
| Rwork | 0.156 |
| R-free | 0.18100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1uwl |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.129 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.800 | 2.060 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.090 | 0.330 |
| Number of reflections | 149793 | |
| <I/σ(I)> | 14.7 | 6.6 |
| Completeness [%] | 97.0 | 90 |
| Redundancy | 6.3 | 5.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.6 | 10% (W/V) PEG8000, 0.2 M MGAC2, 20% (V/V) GLYCERIN, 0.1 M NA CACODYLATE PH 6.9 |
